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首页> 外文期刊>Genome >Cytological and molecular analysis of wheat - Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat
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Cytological and molecular analysis of wheat - Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat

机译：具有普通小麦优良农艺性状的6P染色体片段的小麦-冰ron易位系的细胞学和分子分析。

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摘要

Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat - A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat - A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome. FISH results showed that 15, 5, and 6 lines were translocated onto wheat A, B, and D genomes, respectively. Compared with the previous reports, a fine physical map of 6P chromosome was constructed, consisting of 31 chromosomal bins with 255 STS markers. Twelve translocation lines containing 6PS13 similar to 14 chromosomal bins were highly resistant to leaf rust. Two lines showed high grain number per spike, and three lines displayed both enhanced grain number per spike and thousand-grain weight. Development of wheat - A. cristatum 6P translocation lines will not only provide novel wheat germplasm for wheat breeding but also be helpful to broaden the genetic basis of common wheat.
机译：Agropyron cristatum（2n = 4x = 28，PPPP）是普通小麦的野生近缘种，赋予小麦一些理想的农艺性状，例如高穗粒数和增强的对某些疾病的抵抗力。小麦的开发-广角仙6P易位品系有助于其在小麦改良中的利用。在这项研究中，通过原位杂交（ISH）和6P特异性序列标记位点（STS）标记表征了26条小麦-克里斯塔菌6P易位系。这些易位系携带不同的6P染色体片段，覆盖了整个6P染色体。 FISH结果表明，分别将15、5和6个系转移到小麦A，B和D基因组上。与以前的报告相比，构建了6P染色体的精细物理图谱，该图谱由31个带有255个STS标记的染色体仓组成。包含6PS13的12个易位系与14个染色体箱相似，对叶锈病具有高度抗性。两行显示每个穗粒数高，三行显示每个穗粒数增加和千粒重。小麦的发展-广交会6P易位系不仅为小麦育种提供了新颖的小麦种质，而且有助于拓宽普通小麦的遗传基础。

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