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首页> 外文期刊>Genome >Comparison of genetic diversity betweenCanadian adapted genotypes and exoticgermplasm of soybean
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Comparison of genetic diversity betweenCanadian adapted genotypes and exoticgermplasm of soybean

机译：大豆加拿大适应型基因型与外来种质遗传多样性比较

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Soybean (Glycine max (L.) Men.) was domesticated in China and the greatest genetic diversity for this species is found in Asia. In contrast, in North America, soybean cultivars trace back to a small number of plant introductions from Asia and genetic diversity is typically quite limited. The purpose of this work was to measure and compare the genetic di-versity in two sets of soybean lines. The first set (termed "local") was composed of 100 lines used in a private breeding program in Quebec. The second set (termed "exotic") was composed of 200 lines from elsewhere in the world (but mostly from Asia) and included a few lines of Glycine sofa, the wild progenitor of cultivated soybean. Almost all the genotypes belonged to maturity groups between 000 and II. A total of 39 microsatellites (SSRs) were used to genotype the two col-lections. The number of alleles per locus was almost twice as great in the exotic set compared with the local set. Also, the number of "unique" alleles, i.e., those uniquely present in one set and absent in the other, was almost fivefold greater (191 vs. 37) in a subset of 108 exotic lines with good adaptation than among the local set. A genetic distance matrix, a UPGMA cluster analysis, and a principal coordinate analysis were conducted based on the SSR data. These analyses all indicated that the exotic set was much more diverse and formed a clearly distinct group from the local set. Interestingly, some of the lines showing the best adaptation to local conditions were quite distinctive in terms of their genotype and could potentially contribute useful novel genetic variation within the breeding program.
机译：大豆（Glycine max（L.）Men。）已在中国驯化，该品种的最大遗传多样性在亚洲。相反，在北美，大豆品种可追溯到亚洲少量的植物引种，遗传多样性通常非常有限。这项工作的目的是测量和比较两组大豆品系的遗传多样性。第一组（称为“本地”）由魁北克的一个私人育种计划中使用的100个品系组成。第二组（称为“外来”）由来自世界其他地方（但主要来自亚洲）的200个品系组成，其中包括几行甘氨酸沙发（栽培大豆的野生祖先）。几乎所有基因型都属于000和II之间的成熟度组。共有39个微卫星（SSR）用于对两个集合进行基因分型。在外来环境中，每个基因座的等位基因数量几乎是本地基因组的两倍。而且，在具有良好适应性的108个外来品系的子集中，“独特的”等位基因的数目，即在一组中独特地存在而在另一组中不存在的等位基因的数目几乎比本地组中的多五倍（191对37）。根据SSR数据进行了遗传距离矩阵，UPGMA聚类分析和主坐标分析。这些分析都表明，异国情调的地区更加多样化，并且与当地地区形成了明显不同的群体。有趣的是，一些表现出最佳适应当地条件的品系在基因型方面非常独特，并有可能在育种计划中贡献有用的新颖遗传变异。

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