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首页> 外文期刊>Genome >Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences
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Cytological characterization of sunflower by in situ hybridization using homologous rDNA sequences and a BAC clone containing highly represented repetitive retrotransposon-like sequences

机译：通过使用同源rDNA序列和BAC克隆的原位杂交对向日葵进行细胞学表征，该BAC克隆包含高度代表的重复性反转录转座子样序列

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摘要

In the present work we report new tools for the characterization of the complete chromosome complement of sunflower (Helianthus annuus L.), using a bacterial artificial chromosome (BAC) clone containing repetitive sequences with similarity to retrotransposons and a homologous rDNA sequence isolated from the sunflower genome as probes for FISH. The rDNA signal was found in 3 pairs of chromosomes, coinciding with the location of satellites. The BAC clone containing highly represented retroelements hybridized with all the chromosome complement in FISH, and used together with the rDNA probe allowed the discrimination of all chromosome pairs of sunflower. Their distinctive distribution pattern suggests that these probes could be useful for karyotype characterization and for chromosome identification. The karyotype could be subdivided into 3 clear-cut groups of 12 metacentric pairs, 1 submetacentric pair, and 4 subtelocentric pairs, thus resolving previously described karyotype controversies. The use of BAC clones containing single sequences of specific markers and (or) genes associated with important agricultural traits represents an important tool for future locusspecific identification and physical mapping.
机译：在当前的工作中，我们报告了一种新的工具，用于表征向日葵（Helianthus annuus L.）的完整染色体互补序列，使用了一种细菌人工染色体（BAC）克隆，该克隆包含与逆转座子相似的重复序列以及从向日葵中分离的同源rDNA序列基因组作为FISH的探针。在3对染色体中发现了rDNA信号，这与卫星的位置一致。包含高度代表性的逆转录元件的BAC克隆与FISH中的所有染色体补体杂交，并与rDNA探针一起使用，可以区分向日葵的所有染色体对。它们独特的分布模式表明，这些探针可用于核型鉴定和染色体鉴定。核型可以细分为12个亚中心对，1个亚中心对和4个亚远心对的3个明确的组，从而解决了先前描述的核型争议。使用含有特定标记和（或）与重要农业性状相关的基因的单个序列的BAC克隆，代表了将来进行基因座鉴定和物理作图的重要工具。

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