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首页> 外文期刊>Genome >Expression analysis of defense-related genes in wheat in response to infection by Fusarium graminearum
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Expression analysis of defense-related genes in wheat in response to infection by Fusarium graminearum

机译：禾本科镰刀菌感染对小麦防御相关基因表达的影响

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摘要

Fusarium head blight (FHB), caused by the fungi Fusarium graminearum and Fusarium culmorum, is a worldwide disease of wheat (Triticum aestivum L.). The Chinese cultivar Ning 7840 is one of a few wheat cultivars with resistance to FHB. GeneCalling (TM), an open-architecture mRNA-profiling technology, was used to identify differentially expressed genes induced or suppressed in spikes of Ning 7840 after infection by F. graminearum. One hundred and twenty-five cDNA fragments representing transcripts differentially expressed in wheat spikes were identified. Based on BLASTN and BLASTX analyses, putative functions were assigned to some of the genes: 28 were assigned functions in primary metabolism and photosynthesis, 7 were involved in defense response, 14 were involved in gene expression and regulation, 24 encoded proteins associated with structure and protein synthesis, 42 lacked homology to sequences in the database, and 3 were similar to cloned multidrug resistance or disease resistance proteins. Of particular interest in this study were genes associated with resistance and defense against pathogen infection. Real-time quantitative reverse-transcription PCR indicated that of 51 genes tested, 19 showed 2-fold or greater induction or suppression in infected Ning 7840 in comparison with the water-treated control. The remaining 32 genes were not significantly induced or suppressed in infected Ning 7840 compared with the control. Subsequently, these 19 induced or suppressed genes were examined in the wheat line KS24-1, containing FHB resistance derived from Lophopyrum elongatum, and Len, an FHB-susceptible wheat cultivar. The temporal expression of some of these sequences encoding resistance proteins or defense-related proteins showed FHB (resistance specific) induction, suggesting that these genes play a role in protection against toxic compounds in plant-fungus interactions. On the basis of comprehensive expression profiling of various biotic or abiotic stress response genes revealed by quantitative PCR in this study and other supporting data, we hypothesized that the plant-pathogen interactions may be highly integrated into a network of diverse biosynthetic pathways.
机译：由真菌镰刀镰刀菌和镰刀镰刀菌引起的镰刀菌枯萎病（FHB）是世界范围内的小麦病（Triticum aestivum L.）。中国品种Ning 7840是少数对FHB有抗性的小麦品种之一。 GeneCalling（TM）是一种开放式结构的mRNA分析技术，用于鉴定受到禾谷镰孢菌感染后在Ning 7840穗中诱导或抑制的差异表达基因。鉴定了代表小麦穗中差异表达的转录物的125个cDNA片段。根据BLASTN和BLASTX分析，将推定功能分配给某些基因：28个分配给初级代谢和光合作用的功能，7个参与防御反应，14个参与基因表达和调控，24个编码的蛋白质与结构和结构相关。蛋白质合成中，有42个与数据库中的序列缺乏同源性，还有3个与克隆的耐多药性或抗病性蛋白相似。在这项研究中特别感兴趣的是与抵抗病原体和抵抗病原体感染有关的基因。实时定量逆转录PCR显示，与水处理对照相比，在测试的51个基因中，有19个基因在受感染的Ning 7840中显示出2倍或更大的诱导或抑制。与对照相比，在感染的Ning 7840中未显着诱导或抑制其余32个基因。随后，在小麦品系KS24-1中检测了这19个诱导或抑制的基因，该品系含有来自长双孢镰刀菌（Lophopyrum elongatum）的FHB抗性和对FHB敏感的小麦品种Len（Len）。这些编码抗性蛋白或防御相关蛋白的序列中的某些序列的时间表达显示出FHB（抗性特异性）诱导，表明这些基因在植物-真菌相互作用中对有毒化合物具有保护作用。根据本研究中定量PCR揭示的各种生物或非生物胁迫响应基因的全面表达谱以及其他支持数据，我们假设植物-病原体相互作用可能高度整合到了多种生物合成途径的网络中。

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