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首页> 外文期刊>Genome >Resistance gene analogues are clustered on chromosome 3 of sugar beet and cosegregate with QTL for rhizomania resistance
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Resistance gene analogues are clustered on chromosome 3 of sugar beet and cosegregate with QTL for rhizomania resistance

机译：抗性基因类似物聚集在甜菜的第3号染色体上，并与QTL共同分离，以用于根瘤菌的抗性

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Worldwide, rhizomania is the most important disease of sugar beet. The only way to control this disease is to use resistant varieties. Four full-length resistance gene analogues (RGAs) from sugar beet (cZR-1, cZR-3, cZR-7, and cZR-9) were used in this study. Their predicted polypeptides carry typical nucleotide-binding sites (NBSs) and leucin-rich repeat (LRR) regions, and share high homology to various plant virus resistance genes. Their corresponding alleles were cloned and sequenced from a rhizomania resistant genotype. The 4 RGAs were mapped as molecular markers, using sequence-specific primers to determine their linkage to the rhizomania resistance locus Rz1 in a population segregating for rhizomania resistance. One cZR-3 allele, named Rz-C, together with 5 other molecular markers, mapped to the Rzl locus on chromosome 3 and cosegregated with quantitative trait loci for rhizomania resistance. After screening a bacterial artificial chromosome (BAC) library, 25 cZR-3-positive BACs were identified. Of these, 15 mapped within an interval of approximately 14 cM on chromosome 3, in clusters close to the Rz1 locus. Rz-C differentiates between susceptible and resistant beet varieties, and its transcripts could be detected in all rhizomania resistant varieties investigated. The potential of this RGA marker for cloning of rhizomania resistance genes is discussed.
机译：在世界范围内，根瘤菌病是甜菜最重要的疾病。控制这种疾病的唯一方法是使用抗性品种。在这项研究中使用了四个甜菜的全长抗性基因类似物（RGA）（cZR-1，cZR-3，cZR-7和cZR-9）。他们预测的多肽带有典型的核苷酸结合位点（NBS）和富亮氨酸的重复序列（LRR）区，并且与各种植物病毒抗性基因具有高度同源性。从根瘤菌抗性基因型中克隆了它们的相应等位基因并进行了测序。使用序列特异性引物确定4个RGA作为分子标记，以测定它们与针对根瘤菌抗性隔离的人群中的根瘤菌抗性基因座Rz1的联系。一个名为Rz-C的cZR-3等位基因与其他5个分子标记一起，定位于3号染色体上的Rzl基因座，并与定量特征基因座共分离，以进行根瘤菌抵抗。筛选细菌人工染色体（BAC）文库后，鉴定出25个cZR-3阳性BAC。其中15个位于3号染色体上大约14 cM的间隔内，靠近Rz1基因座。 Rz-C区分易感和抗性甜菜品种，并且其转录本可在所有调查的根瘤菌抗性品种中检测到。讨论了该RGA标记克隆根瘤菌抗性基因的潜力。

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