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首页> 外文期刊>Genome >Detection of interstitial telomeric sequences in the Arctic charr (Salvelinus alpinus) (Teleostei: Salmonidae)
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Detection of interstitial telomeric sequences in the Arctic charr (Salvelinus alpinus) (Teleostei: Salmonidae)

机译：北极鲑（Salvelinus alpinus）（Teleostei：鲑科）间质端粒序列的检测

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摘要

Highly polymorphic Arctic chaff (Salvelinus alpinus Linnaeus, 1758) chromosomes were studied using conventional and molecular methods. The diploid chromosome number in the studied individuals was 2n = 81 or 2n = 82, with a fundamental arm number (NF) = 100. These differences are due to Robertsonian fusions. Interindividual variation in the number and size of DAPI and CMA3 positively stained chromatin sites was observed in studied specimens. In the case of two individuals, the subtelomeric region of the long arm (q) of the largest acrocentric chromosome (chromosome number 10) was positively stained by CMA3 fluorochrome. Both primed in situ labelling (PRINS) and fluorescence in situ hybridization (FISH) revealed that this CMA3-positive region was flanked by telomeric sequences. Previously, the subterminal position of interstitial telomeric sequences located in the vicinity of the CMA3-positive guanine-rich chromatin have been described in two other Salvelinus species, brook trout (Salvelinus fontinalis) and lake trout (Salvelinus namaycush). Moreover, multichromosomal location and variation in size of CMA3 bands have been observed in various Salvelinus taxa, including fishes with internally located telomeric sequences. These results suggest that relocation of CMA3-positive chromatin segments in these species may be facilitated by flanking interstitial telomeric sequences (ITSs).
机译：使用常规和分子方法研究了高度多态的北极谷壳（Salvelinus alpinus Linnaeus，1758）染色体。受研究个体的二倍体染色体数为2n = 81或2n = 82，基本臂数（NF）=100。这些差异是由于罗伯逊融合所致。在研究样本中观察到DAPI和CMA3阳性染色质染色位点数量和大小的个体差异。在两个人的情况下，最大的顶心轴染色体（10号染色体）的长臂（q）的亚端粒区域被CMA3荧光染料阳性染色。引发的原位标记（PRINS）和荧光原位杂交（FISH）均表明该CMA3阳性区域的两侧是端粒序列。以前，已经在另外两个Salvelinus物种（溪鳟（Salvelinus fontinalis）和湖鳟（Salvelinus namaycush））中描述了位于CMA3阳性鸟嘌呤丰富的染色质附近的间质端粒序列的亚末端位置。此外，在各种Salvelinus类群中，包括内部定位端粒序列的鱼类中，均已观察到CMA3带的多染色体定位和大小变化。这些结果表明，侧翼插入间隙端粒序列（ITS）可以促进这些物种中CMA3阳性染色质片段的重定位。

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