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首页> 外文期刊>Biochemistry >Bimodal action of alkaline earth cations on Dictyostelilum discoideum ribonuclease P activity
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Bimodal action of alkaline earth cations on Dictyostelilum discoideum ribonuclease P activity

机译：碱土金属阳离子对Dictyostelilum Discoideum核糖核酸酶P活性的双峰作用

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摘要

The ribonucleoprotein ribonuclease P (RNase P) cleaves all tRNA precursors endonucleolitically to produce the mature 5'-end. Dictyostelium discoideum RNase P displays an absolute requirement for Mg2+. Only the alkaline earth cations Ca2+, Sr2+, and Ba2+, under appropriate conditions can substitute to some extent fur Mg2+. The transition metals Mn2+, Co2+, Ni2+, and Cd2+ are efficient inhibitors of the enzyme activity. Ca2+, Sr2+ and Ba2+, in the presence of Mg2+, exhibit a bimodal action at the kinetic phase of the reaction. Kinetic analysis of the activation phase revealed that Ca2+, Sr2+, or Ba2+ attached on a specific site of RNase P act as nonessential-noncompetitive activators. Further additions of Ca2+, Sr2+, or Ba2+ cause noncompetitive inhibition on the RNase P reaction, indicating that RNase P possesses a second binding site responsible for the inhibitory effect of Ca2+, Sr2+, and Ba2+. Both activator and inhibitory sites can be occupied by Ca2+, Sr2+, or Ba2+ at the same time. [References: 24]
机译：核糖核蛋白核糖核酸酶P（RNase P）会在核酸内切割所有tRNA前体，以产生成熟的5'末端。盘基网柄菌RNase P显示出对Mg2 +的绝对要求。在适当的条件下，只有碱土金属阳离子Ca2 +，Sr2 +和Ba2 +才能在一定程度上替代Mg2 +。过渡金属Mn2 +，Co2 +，Ni2 +和Cd2 +是有效的酶活性抑制剂。在Mg2 +存在下，Ca2 +，Sr2 +和Ba2 +在反应的动力学阶段表现出双峰作用。活化阶段的动力学分析表明，附着在RNase P特定位点上的Ca2 +，Sr2 +或Ba2 +充当非竞争性非竞争性激活剂。进一步添加Ca2 +，Sr2 +或Ba2 +会导致对RNase P反应的非竞争性抑制，表明RNase P具有第二个结合位点，负责Ca2 +，Sr2 +和Ba2 +的抑制作用。激活位点和抑制位点都可以同时被Ca2 +，Sr2 +或Ba2 +占据。 [参考：24]

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