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Effects of Akt signaling on nuclear reprogramming

机译：Akt信号转导对核重编程的影响

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摘要

Reprogramming of the epigenetic state from differentiated to pluripotent cells can be attained by cell fusion of differentiated somatic cells with embryonic stem (ES) cells or transfer of the nucleus of a differentiated cell into an enucleated oocyte. Activation of Akt signaling is sufficient to maintain pluripotency of ES cells and promotes derivation of embryonic germ (EG) cells from primordial germ cells (PGCs). Here we analyzed the effects of Akt signaling on somatic cell nuclear reprogrammingafter cell fusion and nuclear transfer. We found that forced activation of Akt signaling stimulated reprogramming after cell fusion of ES cells with thymocytes or mouse embryonic fibroblasts. These hybrid cells showed ES cell characteristics, including in vitro and in vivo differentiation capacity. In contrast, Akt signaling significantly reduced the efficiency of reprogramming with nuclear transfer. Our results demonstrate that Akt signaling plays important roles on the nuclear reprogramming of somaticcells.
机译：通过将分化的体细胞与胚胎干（ES）细胞进行细胞融合或将分化细胞的核转移到去核卵母细胞中，可以实现将表观遗传状态从分化细胞重新编程为多能细胞。 Akt信号传导的激活足以维持ES细胞的多能性，并促进从原始生殖细胞（PGC）衍生出胚胎生殖（EG）细胞。在这里，我们分析了Akt信号转导对细胞融合和核转移后体细胞核重编程的影响。我们发现，ES细胞与胸腺细胞或小鼠胚胎成纤维细胞融合后，Akt信号传导的强制激活刺激了重编程。这些杂种细胞表现出ES细胞特征，包括体外和体内分化能力。相反，Akt信号转导显着降低了核转移重新编程的效率。我们的结果表明，Akt信号传导在体细胞的核重编程中发挥重要作用。

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