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首页> 外文期刊>Genes to cells : >Activation of facultatively silenced Drosophila loci associates with increased acetylation of histone H2AvD.
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Activation of facultatively silenced Drosophila loci associates with increased acetylation of histone H2AvD.

机译：兼性沉默的果蝇基因座的激活与组蛋白H2AvD乙酰化的增加有关。

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摘要

H2A.Z is an evolutionarily highly conserved non-allelic variant of histone H2A. H2A.Z and its homologues have been shown to involve in both chromatin silencing and activation. Although much of our knowledge of H2A.Z biological activity has come from studies on its yeast homologue Htz1, H2A.Z appears to have more complex and diverse functions in higher eukaryotes. To investigate the involvement of H2AvD, a Drosophila homologue of mammalian H2A.Z, in mechanisms of conditional activation of facultatively silenced genes, we generated transgenic Drosophila lines expressing H2AvD fused at the C- or N-terminus with the green fluorescent protein (GFP). Using heat shock-induced gene activation and polytene chromosome puff formation as an in vivo model system, we analyzed effects of H2AvD termini modifications on transcription. We found that N-terminally fused GFP inhibited H2AvD acetylation and impaired heat shock-induced puff formation and hsp70 gene activation. Our data suggest that the N-terminal region of H2AvD plays a pivotal role in transcriptional activation and that induction of transiently silenced Drosophila loci associates with increased acetylation of H2AvD.
机译：H2A.Z是组蛋白H2A的进化高度保守的非等位基因变体。 H2A.Z及其同系物已被证明参与染色质沉默和激活。尽管我们对H2A.Z生物活性的许多了解来自其酵母同系物Htz1的研究，但H2A.Z在高等真核生物中似乎具有更复杂和多样的功能。为了研究哺乳动物H2A.Z的果蝇同源物H2AvD在兼性沉默基因的条件激活机制中的参与，我们产生了表达H2AvD融合在其上的转基因果蝇品系。 C或N末端带有绿色荧光蛋白（GFP）。使用热休克诱导的基因激活和多聚体染色体膨化形成作为 in vivo 模型系统，我们分析了H2AvD末端修饰对转录的影响。我们发现，N末端融合的GFP抑制H2AvD乙酰化，并损害热激诱导的粉扑形成和 hsp70 基因激活。我们的数据表明，H2AvD的N端区域在转录激活中起着关键作用，而瞬时沉默的果蝇基因座的诱导与H2AvD乙酰化的增加有关。

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