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首页> 外文期刊>Genome >Comparison of the chromosome maps around a resistance hot spot on chromosome 5 of potato and tomato using BAC-FISH painting
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Comparison of the chromosome maps around a resistance hot spot on chromosome 5 of potato and tomato using BAC-FISH painting

机译：使用BAC-FISH绘画比较马铃薯和番茄5号染色体上抗性热点周围的染色体图

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摘要

Potato chromosome 5 harbours numerous genes for important qualitative and quantitative traits, such as resistance to the root cyst nematode Globodera pallida and the late blight fungus, Phytophthora infestans. The genes make up part of a "hot spot" for resistances to various pathogens covering a genetic map length of 3 cM between markers GP21 and GP179. We established the physical size and position of this region on chromosome 5 in potato and tomato using fluorescence in situ hybridization (FISH) on pachytene chromosomes. Five potato bacterial artificial chromosome (BAC) clones with the genetically anchored markers GP21, Rl-contig (proximal end), CosA, GP179, and StPto were selected, labeled with different fluorophores, and hybridized in a five-colour FISH experiment. Our results showed the location of the BAC clones in the middle of the long arm of chromosome 5 in both potato and tomato. Based on chromosome measurements, we estimate the physical size of the GP21–GP179 interval at 0.85 Mb and 1.2 Mb in potato and tomato, respectively. The GP21–GP179 interval is part of a genome segment known to have inverted map positions between potato and tomato.
机译：马铃薯5号染色体具有许多重要的定性和定量性状基因，例如对根孢囊线虫Globodera pallida和晚疫病真菌疫霉疫霉具有抗性。这些基因构成了对各种病原体的抗性“热点”的一部分，涵盖了标记GP21和GP179之间3 cM的遗传图谱长度。我们使用粗线染色体上的荧光原位杂交（FISH）在马铃薯和番茄中的5号染色体上确定了该区域的物理大小和位置。选择五个具有遗传锚定标记GP21，Rl-contig（近端），CosA，GP179和StPto的马铃薯细菌人工染色体（BAC）克隆，用不同的荧光团标记，并在五色FISH实验中杂交。我们的结果表明，BAC克隆在马铃薯和番茄中均位于5号染色体长臂的中间。根据染色体测量，我们估计在马铃薯和番茄中，GP21-GP179区间的物理大小分别为0.85 Mb和1.2 Mb。 GP21–GP179区间是基因组片段的一部分，已知该片段在马铃薯和番茄之间具有反向的图位。

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