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首页> 外文期刊>Genes to cells : >Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex.
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Recruitment of CG-NAP to the Golgi apparatus through interaction with dynein-dynactin complex.

机译：通过与动力蛋白-动力蛋白复合物的相互作用将CG-NAP招募到高尔基体。

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摘要

The structural organization and position of the Golgi apparatus are highly regulated by microtubule cytoskeleton and microtubule motor proteins. The mechanisms linking these proteins to the Golgi apparatus remain elusive. Here, we found that centrosome and Golgi-localized PKN associated protein (CG-NAP) was localized to the Golgi apparatus in a microtubule-dependent manner. Microtubule-binding experiments revealed that CG-NAP possessed two microtubule-binding domains. We also found that CG-NAP was well co-localized with cytoplasmic dynein subunits during recovery from the on-ice treatment of cells that induced dissociation of CG-NAP from the Golgi. Similar co-localization was observed during recovery from the acetate treatment, which has been reported to inhibit the dynein-mediated transport. CG-NAP was co-immunoprecipitated with a dynactin subunit p150(Glued). Expressing the p150(Glued)-binding region of CG-NAP fused with mitochondria-targeting sequence induced recruitment of mitochondria to the pericentriolar area, suggesting that this region interacts with functional cytoplasmic dynein in vivo. Moreover, over-expression of this region caused fragmentation of the Golgi similar to that of dynamitin. These results suggest that CG-NAP is recruited to the minus ends of microtubules by interacting with cytoplasmic dynein, thereby localizes to the Golgi apparatus in a microtubule-dependent manner and possibly involved in the formation of the Golgi near the centrosomes.
机译：高尔基体的结构组织和位置受到微管细胞骨架和微管运动蛋白的高度调节。将这些蛋白质与高尔基体连接的机制仍然难以捉摸。在这里，我们发现中心体和高尔基体定位的PKN相关蛋白（CG-NAP）以微管依赖的方式定位于高尔基体。微管结合实验表明，CG-NAP具有两个微管结合域。我们还发现，在从冰上处理诱导CG-NAP从高尔基体解离的细胞中恢复期间，CG-NAP与细胞质动力蛋白亚基共定位良好。从醋酸盐处理过程中观察到类似的共定位现象，据报道抑制了动力蛋白介导的转运。 CG-NAP与Dynactin亚基p150（Glued）共免疫沉淀。表达与线粒体靶向序列融合的CG-NAP的p150（Glued）结合区域可诱导线粒体募集到中心小肠周围区域，提示该区域在体内与功能性细胞质动力蛋白相互作用。此外，该区域的过度表达引起高尔基的分裂，类似于dynamitin。这些结果表明，CG-NAP通过与细胞质动力蛋白相互作用而被募集至微管的负端，从而以微管依赖性的方式定位于高尔基体，并且可能参与了靠近中心体的高尔基体的形成。

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