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首页> 外文期刊>Genes to cells : >Post-transcriptionally generated cell heterogeneity regulates biofilm formation in Bacillus subtilis
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Post-transcriptionally generated cell heterogeneity regulates biofilm formation in Bacillus subtilis

机译：转录后产生的细胞异质性调节枯草芽孢杆菌中的生物膜形成

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摘要

Bacillus subtilis forms biofilms in appropriate environments by producing extracellular matrices. Genes required for matrix formation, for example tapA, are regulated by the SinI/SinR/SlrR system. SinR is the repressor for tapA. SinI and SlrR inhibit DNA-binding of SinR. sinI and sinR constitute two-gene operon, and sinR has its own promoter. During biofilm formation, a portion of the population differentiates into matrix-producing cells. This is thought to be caused by Spo0A-dependent, heterogeneous expression of the PsinI promoter, whereas the PsinR promoter is expressed homogeneously. However, we observed that at its original locus, overall sinI transcription was almost homogeneous, because upstream read-through transcription from PyqHG would overcome expression of PsinI. When we used translational sinI-gfp and sinR-mCherry reporters at their original loci, their fluorescence distribution patterns in the cell population were clearly bimodal. This bimodal expression might be caused by cell-to-cell variations of mRNA stability. This study shows that the post-transcriptionally regulated bimodal expression of SinI and SinR is important for bacterial cell-fate determination.
机译：枯草芽孢杆菌通过产生细胞外基质在适当的环境中形成生物膜。基质形成所需的基因，例如tapA，由SinI / SinR / SlrR系统调控。 SinR是tapA的阻遏物。 SinI和SlrR抑制SinR的DNA结合。 sinI和sinR构成两个基因的操纵子，而sinR具有其自己的启动子。在生物膜形成过程中，一部分种群分化为产生基质的细胞。认为这是由PsinI启动子的Spo0A依赖性异源表达引起的，而PsinR启动子却被均一表达。但是，我们观察到，在其原始基因座上，总体sinI转录几乎是同质的，因为从PyqHG进行的上游通读转录将克服PsinI的表达。当我们在原始位点使用翻译的sinI-gfp和sinR-mCherry报告基因时，它们在细胞群体中的荧光分布模式显然是双峰的。这种双峰表达可能是由mRNA稳定性的细胞间变化引起的。这项研究表明，转录后调控的SinI和SinR的双峰表达对于细菌细胞命运的确定很重要。

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