• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genes to cells : >Novel pathway directed by sigma(E) to cause cell lysis in Escherichia coli
【24h】

Novel pathway directed by sigma(E) to cause cell lysis in Escherichia coli

机译：由sigma（E）引导导致大肠杆菌细胞裂解的新型途径

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A large number of Escherichia coli cells become viable but nonculturable at early stationary phase, most of which are directed to lysis in cells with an enhanced active sE level. In this study, we examined the effect of small noncoding RNAs, MicA and RybB, as sE regulon as well as regulators of outer membrane protein (Omp) genes, on the lysis process. micA- and rybB-disrupted mutations almost completely suppressed the cell lysis. Increased expression of micA and rybB or disrupted mutation of ompA, ompC and ompW led to a significant level of cell lysis. The suppression by Mg2+ was found to maintain the integrity of the Omp-repressed outer membrane. Taken together, the results suggest that the cell lysis proceeds in the cascade of sE ? expression of micA and rybB ? reduction in Omp proteins ? disintegration of the outer membrane.
机译：大量大肠杆菌细胞在早期固定阶段就变得可行，但无法培养，其中大多数针对的是在具有较高活性sE水平的细胞中裂解。在这项研究中，我们检查了小的非编码RNA，MicA和RybB，sE调节子以及外膜蛋白（Omp）基因的调节剂对裂解过程的影响。 micA和rybB破坏的突变几乎完全抑制了细胞裂解。 micA和rybB的表达增加或ompA，ompC和ompW的突变破坏导致细胞裂解水平显着提高。发现通过Mg 2+的抑制保持了Omp抑制的外膜的完整性。两者合计，结果表明细胞裂解以sE？级联进行。 micA和rybB的表达？减少Omp蛋白？外膜崩解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号