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首页> 外文期刊>Genome >Mapping of a major locus controlling seed dormancy using backcrossed progenies in wheat (Triticum aestivum L.)
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Mapping of a major locus controlling seed dormancy using backcrossed progenies in wheat (Triticum aestivum L.)

机译：使用回交后代的小麦（Triticum aestivum L.）控制种子休眠的主要基因座图谱

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Seed dormancy is an important factor regulating preharvest sprouting (PHS) but is a complex trait for genetic analysis. We previously identified a major quantitative trait locus (QTL) controlling seed dormancy on the long arm of chromosome 4A (4AL) in common wheat. To transfer the QTL from the dormant lines ‘OS21-5’ and ‘Leader’ into the Japanese elite variety ‘Haruyokoi’, which has an insufficient level of seed dormancy, backcrossing was carried out through marker-assisted selection (MAS) using PCR-based codominant markers. Nineteen BC_5F_2 plants with homozygous alleles of ‘OS21-5’ or ‘Haruyokoi’ were developed and evaluated for seed dormancy under greenhouse conditions. The seeds harvested from plants with ‘OS21-5’ alleles showed a clearly high level of dormancy compared with seeds from plants with ‘Haruyokoi’ alleles. Additionally, the dormancy phenotype of BC_3F_3 seeds harvested from 128 BC_3F_2 plants with homozygous alleles of ‘Leader’ or ‘Haruyokoi’ showed a clear difference between these alleles. The QTL on 4AL confers a major gene, Phs1, which was mapped within a 2.6 cM region. The backcrossed lines developed in this study can be important sources for improving PHS resistance in Japanese wheat and for analyzing the mechanism of seed dormancy. MAS was useful for the development of near-isogenic lines in this complex trait, to facilitate the molecular dissection of genetic factors.
机译：种子休眠是调节收获前发芽（PHS）的重要因素，但是遗传分析的复杂性状。我们之前在普通小麦中确定了一个主要的定量性状基因座（QTL），用于控制4A号染色体（4AL）长臂上的种子休眠。为了将QTL从休眠系'OS21-5'和'Leader'转移到种子休眠水平不足的日本优良品种'Haruyokoi'，通过使用PCR-的标记辅助选择（MAS）进行回交基于共性标记。开发了19个BC_5F_2等位基因为'OS21-5'或'Haruyokoi'的纯合植物，并在温室条件下评估了种子休眠的能力。与具有“ Haruyokoi”等位基因的植物的种子相比，具有“ OS21-5”等位基因的植物的种子显示出明显较高的休眠水平。此外，从具有“ Leader”或“ Haruyokoi”纯合等位基因的128 BC_3F_2植物中收获的BC_3F_3种子的休眠表型显示了这些等位基因之间的明显差异。 4AL上的QTL赋予一个主要基因Phs1，该基因被定位在2.6 cM区域内。本研究开发的回交系可能是提高日本小麦对PHS的抗性以及分析种子休眠机制的重要来源。 MAS可用于开发这种复杂性状的近等基因系，以促进遗传因素的分子解剖。

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