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首页> 外文期刊>Genetics and molecular biology: publication of the Sociedade Brasileira de Genetica >A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections
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A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections

机译：从博物馆藏品的研究皮肤中提取和序列分析DNA的简单协议

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摘要

Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64% of 64 skin samples from three-toed sloths (Bradypus vadegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of S?o Paulo Museum of Zoology (Museu de Zoologiada Universidadede S?o Paulo, MUZUSP) S?o Paulo, S?o Paulo, Brazil; 18 samples from the Emílio Goeldi Museum (Museu Paraense Emílio Goeldi, MPEG), Belém, Pará, Brazil; and 3 samples from the Museum of Vertebrate Zoology (MVZ) University of California, Berkeley, USA. The specimens sampled ranged in age from 18 to 108 years old. Our methodology allowed the recovery of up to 700 bp of mitochondrial DNA and 400 bp of nuclear genes. Thereafter, it is useful for genetic diversity studies of three-toed sloths and could be applied to other animals.
机译：博物馆的藏品已被广泛用作分子生物学研究的生物样品来源，并且有多种方法和技术可从博物馆中存档的组织中获取和分析DNA，但是其中大多数协议都是针对特定组织而开发的，或者是商业套件。我们提出了从树懒博物馆标本中提取和扩增DNA片段的简单协议。通过这个简单的协议，我们分析了在三个不同的博物馆中保存的三趾树懒（Bradypus vadegatus和Bradypus tridactylus）的64种皮肤样本中64％的DNA片段：来自圣保罗大学动物学博物馆的43种样本MUZUSP，圣保罗大学）巴西圣保罗，圣保罗;来自巴西帕拉贝伦的EmílioGoeldi博物馆（Museu ParaenseEmílioGoeldi，MPEG）的18个样本;以及来自美国伯克利加利福尼亚大学脊椎动物生态博物馆（MVZ）的3个样本。样本的年龄范围为18至108岁。我们的方法可以回收多达700 bp的线粒体DNA和400 bp的核基因。此后，它对三趾树懒的遗传多样性研究很有用，并可应用于其他动物。

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