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首页> 外文期刊>Genomics >Molecular cloning, chromosomal mapping, and characteristic expression in tooth organ of rat and mouse Krox-25.
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Molecular cloning, chromosomal mapping, and characteristic expression in tooth organ of rat and mouse Krox-25.

机译：大鼠和小鼠Krox-25的分子克隆，染色体作图以及在牙齿器官中的特征性表达。

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摘要

A novel member of the Krox family of proteins, designated Krox-25, was identified by screening clones from the cDNA libraries of a rat incisor and mouse embryo craniofacial tissue. Rat and mouse Krox-25 mRNAs are about 2.4 kb long, encoding 225 and 224 amino acids, respectively. Krox-25 consists of five zinc finger motifs homologous to the Drosophila Kruppel segmentation gene and also contains several consensus amino acid sequences for a protein kinase C binding domain. Northern blot analysis revealed an intense expression of Krox-25 mRNA in rat and mouse teeth, although it was expressed weakly in other tissues, including calvaria, brain, lung, thymus, kidney, and submandibular gland of mouse. In situ hybridization showed that Krox-25 mRNA began to be expressed weakly in the early odontogenic mesenchyme and primitive enamel epithelium located at the apical end of the rat incisor, and Krox-25 expression increased in the presecretory ameloblasts and became intense in the secretory ameloblasts. This expression was also similar to the results of immunohistochemistry and Western blot, especially the Krox-25 localization in the nuclei of enamel epithelial cells. These results suggest that Krox-25 plays an important role as a transcription factor for the cytodifferentiation and amelogenesis of enamel epithelium.
机译：通过筛选来自大鼠门齿和小鼠胚胎颅面组织的cDNA文库中的克隆，鉴定出了Krox家族的一个新成员，命名为Krox-25。大鼠和小鼠Krox-25 mRNA的长度约为2.4 kb，分别编码225和224个氨基酸。 Krox-25由与果蝇Kruppel分割基因同源的五个锌指基序组成，并且还包含蛋白激酶C结合域的几个共有氨基酸序列。 Northern印迹分析揭示了Krox-25 mRNA在大鼠和小鼠牙齿中的强烈表达，尽管在其他组织中表达较弱，包括小鼠的颅骨，脑，肺，胸腺，肾脏和下颌下腺。原位杂交表明，Krox-25 mRNA在大鼠门齿顶端的早期牙源性间充质和原始釉质上皮中开始弱表达，Krox-25在分泌前成釉细胞中表达增加，在分泌性成釉细胞中表达增强。 。该表达也与免疫组织化学和蛋白质印迹的结果相似，尤其是在釉质上皮细胞核中的Krox-25定位。这些结果表明，Krox-25作为釉质上皮细胞分化和牙釉质形成的转录因子起着重要作用。

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