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Applications of mass spectrometry techniques in the investigation of milk proteome

机译：质谱技术在牛奶蛋白质组学研究中的应用

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摘要

The introduction of "soft" desorption/ionization methods such as electrospray ionization and matrix-assisted laser desorption/ionization has determined a breakthrough in the application of mass spectrometry to the structural analysis of proteins. The contemporary advancement of bioinformatics, together with the possibility to combine these mass spectrometric methods with electrophoretic or chromatographic separation techniques has opened up the new field of proteome analysis and, more generally, has established these approaches as indispensable tools for protein and peptide analysis in complex mixtures, such as milk and milk-derived foods. Here, a necessarily not exhaustive series of current applications of mass spectrometry-based techniques for the characterization of milk proteins will be summarized. These include the characterization of milk protein polymorphism, determination of the structural modifications induced on milk proteins by industrial processes, investigation of milk adulterations and characterization of milk allergens.
机译：诸如电喷雾电离和基质辅助激光解吸/电离之类的“软”解吸/电离方法的引入，已经确定了质谱在蛋白质结构分析中的应用突破。生物信息学的当代发展，以及将这些质谱方法与电泳或色谱分离技术相结合的可能性，开辟了蛋白质组学分析的新领域，并且更广泛地将这些方法确立为复杂蛋白质和肽段分析不可或缺的工具混合物，例如牛奶和牛奶来源的食物。在此，将对基于质谱技术的牛奶蛋白质表征的当前应用的未必详尽的系列进行总结。这些包括乳蛋白多态性的表征，通过工业过程对乳蛋白诱导的结构修饰的确定，乳掺假的研究以及乳过敏​​原的表征。

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