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首页> 外文期刊>European journal of mass spectrometry >Matrix-assisted laser desorption/ionization post-source decay fragmentation of the cystine-containing amphibian peptides with novel cysteine tags
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Matrix-assisted laser desorption/ionization post-source decay fragmentation of the cystine-containing amphibian peptides with novel cysteine tags

机译：具有新型半胱氨酸标签的含半胱氨酸的两栖动物肽的基质辅助激光解吸/电离源衰变后的断裂

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摘要

Long disulfide-containing peptides, brevinins 1E and 2Ec, from the skin secretion of the frog Rana ridibunda were reduced and alkylated with ten novel and three known derivatizing agents. Nine of novel reagents are maleimide derivatives. The peptides were also reduced with dithiothreitol directly on a matrix-assisted laser desorption/ionization (MALDI) target without alkylation. Modified samples were subjected to a MALDI-post-source decay study. Procedures, fragmentation patterns, fragment ion signal abundances and sequence coverage for two peptides modified with 13 tags (or on-plate reduced) are described. The fast on-plate procedure for reduction/alkylation was applied to a crude secretion from Rana ridibunda, providing intense signals of derivatized peptides. The corresponding ions may be used for the tandem mass spectrometry sequencing procedure.
机译：来自蛙蛙蛙的皮肤分泌物的长的含二硫键的短素，短素1E和2Ec被还原并用十种新的和三种已知的衍生剂烷基化。九种新试剂是马来酰亚胺衍生物。肽也可以直接在基质辅助的激光解吸/电离（MALDI）靶标上用二硫苏糖醇还原而无需烷基化。修改后的样品进行了MALDI后源衰减研究。描述了用13个标签修饰（或在板上进行还原）修饰的两种肽的程序，片段化模式，片段离子信号丰度和序列覆盖率。快速还原/烷基化的板载程序应用于来自蛙蛙的粗分泌物，提供了强烈的衍生肽信号。相应的离子可用于串联质谱测序程序。

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