Positive-ion fragmentation in matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O : 1.

Kovacik V; Bekesova S; Patoprsty V; Rehulka P; Chmelik J; Kovac P

首页> 外文期刊>European journal of mass spectrometry >Positive-ion fragmentation in matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O : 1.

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Positive-ion fragmentation in matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O : 1.

机译：霍乱弧菌O特异性O多糖的合成类似物在基质辅助激光解吸/电离串联飞行时间质谱中的正离子裂解。

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Saccharides (mono- to hexamers) that mimic the terminal epitopes of O-antigens of Vibrio cholerae O:1, serotypes Ogawa and Inaba have been studied by matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI ToF/ToF) mass spectrometry (MS). Cationized adducts are the characteristic ions formed through the capture of sodium or potassium cations under MALDI MS conditions. Three characteristic pathways dominate in the fragmentation of model substances under MALDI ToF/ToF collision-induced dissociation MS/MS conditions of measurement. The first is the elimination which shortens the length of the oligosaccharide. In this way, conversion of the Ogawa to Inaba fragments takes place under the conditions of measurement. In the second, the conjugated transfer of electrons in the upstream unit of oligosaccharides takes place. The third route brings about the elimination of one alpha-hydroxy-gamma-butyrolactone molecule from the 4-(3-deoxy-L-glycero-tetronamido) group. The MALDI ToF/ToF MS/MS provided sufficient information about molecular mass, the number of saccharide residues, the structure of saccharides, the C(4)- amide of 3-deoxy-L-glycero-tetronic acid (DGT) of the compounds investigated and allows Ogawa and Inaba serotypes to be distinguished.

机译：通过基质辅助激光解吸/电离串联飞行时间（MALDI ToF / ToF）研究了模拟霍乱弧菌O：1 O型抗原，血清型Ogawa和Inaba的糖精（单聚体或六聚体）质谱（MS）。阳离子加合物是在MALDI MS条件下通过捕获钠或钾阳离子形成的特征离子。在MALDI ToF / ToF碰撞诱导的解离MS / MS测量条件下，模型物质的碎裂中，三个特征途径占主导地位。首先是消除，它缩短了寡糖的长度。这样，在测量条件下将小川碎片转化为稻叶碎片。第二，在寡糖的上游单元中发生电子的共轭转移。第三种途径消除了4-（3-脱氧-L-甘油-四氢酰胺基）基团中的一个α-羟基-γ-丁内酯分子。 MALDI ToF / ToF MS / MS提供了有关化合物的分子量，糖残基数，糖结构，化合物的3-脱氧-L-甘油-tetronic酸（DGT）的C（4）-酰胺的足够信息调查并区分小川和稻叶的血清型。

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《European journal of mass spectrometry》 |2006年第4期|共6页
作者
Kovacik V; Bekesova S; Patoprsty V; Rehulka P; Chmelik J; Kovac P;
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正文语种 eng
中图分类分析化学;
关键词
matrix assisted laser desorption/ionization time of flight/time of flight; MALDI ToF/ToF positive tandem mass spectra; oligosaccharides from lipopolysaccharide of Vibrio cholerae O : 1; METHYL ALPHA-GLYCOSIDES; SEROTYPE-OGAWA; ANTIGEN; INABA; LIPOPOLYSACCHARIDE; VIBRIO-CHOLERAE-O-1; TRISACCHARIDE; ELECTROSPRAY; DISACCHARIDE; MIMICKING;

机译：基质辅助激光解吸/电离飞行时间/飞行时间;MALDI ToF / ToF正串联质谱;霍乱弧菌O的脂多糖中的寡糖：1;甲基α-糖苷;SEROTYPE-OGAWA;ANTIGEN;INABA;LIPOPOLYSACCHARIDE;VIBRIO- CHOLERAE-O-1;三糖;电子喷雾;二糖;模拟;

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1. Positive-ion fragmentation in matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O : 1. [J] . Kovacik V, Bekesova S, Patoprsty V, European journal of mass spectrometry . 2006,第4期

机译：霍乱弧菌O特异性O多糖的合成类似物在基质辅助激光解吸/电离串联飞行时间质谱中的正离子裂解。
2. Elimination of isocyanate and isothiocyanate molecules at the electrospray ionization ion trap, electrospray ionization quadrupole time-of-flight and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry fragmentation of [J] . Slavka Bekesova, Vladimir Kovacik, Igor Tvaroska, European journal of mass spectrometry . 2007,第2期

机译：消除了电喷雾电离离子阱处的异氰酸酯和异硫氰酸酯分子，电喷雾电离四极杆飞行时间和基质辅助激光解吸/电离飞行时间质谱联用
3. In-Source Decay and Pseudo Tandem Mass Spectrometry Fragmentation Processes of Entire High Mass Proteins on a Hybrid Vacuum Matrix-Assisted Laser Desorption Ionization-Quadrupole Ion-Trap Time-of-Flight Mass Spectrometer [J] . Lyna Sellami, Omar Belgacem, Claude Villard, Analytical chemistry . 2012,第12期

机译：混合真空基质辅助激光解吸电离-四极杆离子阱飞行时间质谱仪上全部高质量蛋白质的源内衰减和伪串联质谱裂解过程
4. Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectromet [C] . Daisy-Malloy Hamburg, Moo-Jin Suh, Patrick A. Limbach International Symposium on Runaway Reactions, Pressure Relief Design and Effluent Handling American Institute of Chemical Engineers/American Chemical Society Management Conference . 2005

机译：通过基质辅助激光解吸/电离质谱法通过基质辅助激光解吸/电离质谱鉴定通过基质辅助激光解吸/电离质谱鉴定蛋白质中的蛋白质中蛋白质的鉴定
5. Some quantitative aspects of the analysis of synthetic polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [D] . Goldschmidt, Robert J. 1998

机译：基质辅助激光解吸/电离飞行时间质谱分析合成聚合物的一些定量方面
6. Multiple Time-of-Flight/Time-of-Flight Events in a Single Laser Shot for Improved Matrix-Assisted Laser Desorption/Ionization Tandem Mass Spectrometry Quantification [O] . Boone M. Prentice, Chad W. Chumbley, Brian C. Hachey, -1

机译：单次激光发射中的多个飞行时间/飞行时间事件用于改进的基质辅助激光解吸/电离串联质谱定量
7. Characterization of gallotannins from Astronium species by flow injection analysis-electrospray ionization-ion trap-tandem mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry [O] . Viviane Candida da Silva, Assunta Napolitano, Daniela Eletto, 2011

机译：通过流动注射分析 - 电喷雾离子化 - 离子阱 - 串联质谱和基质辅助激光解吸/电离飞行时间质谱对来自天文物种的gall单宁进行表征

Positive-ion fragmentation in matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry of synthetic analogs of the O-specific polysaccharide of Vibrio cholerae O : 1.

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