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首页> 外文期刊>European journal of mass spectrometry >Mass spectrometric approaches for elucidation of antigen–antibody recognition structures in molecular immunology
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Mass spectrometric approaches for elucidation of antigen–antibody recognition structures in molecular immunology

机译：在分子免疫学中阐明抗原-抗体识别结构的质谱方法

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摘要

Mass spectrometric approaches have recently gained increasing access to molecular immunology and several methods have been developed that enable detailed chemical structure identification of antigen?antibody interactions. Selective proteolytic digestion and MS-peptide mapping (epitope excision) has been successfully employed for epitope identification of protein antigens. In addition, “affinity proteomics” using partial epitope excision has been developed as an approach with unprecedented selectivity for direct protein identification from biological material. The potential of these methods is illustrated by the elucidation of a β- amyloid plaque-specific epitope recognized by therapeutic antibodies from transgenic mouse models of Alzheimer抯 disease. Using an immobilized antigen and antibody-proteolytic digestion and analysis by high resolution Fourier transform ion cyclotron resonance mass spectrometry has lead to a new approach for the identification of antibody paratope structures (paratope-excision; “parexprot”). In this method, high resolution MS-peptide data at the low ppm level are required for direct identification of paratopes using protein databases. Mass spectrometric epitope mapping and determination of “molecular antibody-recognition signatures” offer high potential, especially for the development of new molecular diagnostics and the evaluation of new vaccine lead structures.
机译：质谱方法最近获得了越来越多的分子免疫学途径，并且已经开发了几种方法，可以对抗原-抗体相互作用进行详细的化学结构鉴定。选择性蛋白水解消化和MS肽作图（表位切除）​​已成功用于蛋白质抗原的表位鉴定。此外，已经开发出使用部分表位切除的“亲和蛋白质组学”作为一种从生物材料中直接鉴定蛋白质具有前所未有的选择性的方法。这些方法的潜力通过阐明被阿尔茨海默氏病转基因小鼠模型的治疗性抗体识别的β-淀粉样蛋白斑块特异性表位得以阐明。使用固定的抗原和抗体蛋白水解消化以及通过高分辨率傅立叶变换离子回旋共振质谱进行分析，已导致鉴定抗体对位结构（对位切除;“ parexprot”）的新方法。在此方法中，需要使用低ppm水平的高分辨率MS肽数据才能使用蛋白质数据库直接鉴定对位。质谱表位作图和“分子抗体识别标记”的确定具有很高的潜力，特别是在开发新的分子诊断方法和评估新的疫苗前导结构方面。

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