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首页> 外文期刊>Genomics >Human eIF5A2 on chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression.
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Human eIF5A2 on chromosome 3q25-q27 is a phylogenetically conserved vertebrate variant of eukaryotic translation initiation factor 5A with tissue-specific expression.

机译：染色体3q25-q27上的人类eIF5A2是具有组织特异性表达的真核翻译起始因子5A的系统进化保守脊椎动物变异体。

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摘要

Eukaryotic translation initiation factor 5A (eIF5A) is an essential protein tightly linked to cellular polyamine homeostasis. It receives the unique spermidine-derived posttranslational modification hypusine that is necessary for eIF5A's biochemical activity and cellular proliferation. The eIF5A protein stimulates ribosomal peptidyl-transferase and may be involved in nucleocytoplasmic mRNA transport. Little is known about the molecular genetics of eIF5A. Here we report on the sequence and molecular characterization of human EIF5A2, a novel phylogenetically conserved gene for eIF5A. EIF5A2 stretches over 17 kb and consists of five exons and four introns. It is localized at 3q25-q27, often noted for chromosomal instability in cancers. EIF5A2 is highly expressed in testis and colorectal adenocarcinoma and at moderate levels in the brain, in contrast to the ubiquitously expressed EIF5A1 gene. Two EIF5A2 mRNAs share a 129-nt 5' UTR and a coding sequence for the 153-amino-acid eIF5AII protein, but possess two alternative 3' UTRs of 46 and 890 nt that arise through differential polyadenylation. The protein is 84% identical and 94% similar to eIF5AI. Both EIF5A genes are conserved in vertebrates. Our findings lend further support for a specialized gene expression program of polyamine metabolic proteins and regulators that function to maintain polyamine homeostasis at elevated levels during spermatogenesis. Copyright 2001 Academic Press.
机译：真核翻译起始因子5A（eIF5A）是与细胞多胺体内平衡紧密相连的必需蛋白。它接受了独特的亚精胺衍生的翻译后修饰hy，这对于eIF5A的生化活性和细胞增殖是必不可少的。 eIF5A蛋白刺激核糖体肽基转移酶，可能参与核质mRNA的运输。关于eIF5A的分子遗传学知之甚少。在这里，我们报告人类EIF5A2，eIF5A的一个新的系统发育保守基因的序列和分子表征。 EIF5A2延伸超过17 kb，由五个外显子和四个内含子组成。它位于3q25-q27，通常在癌症中引起染色体不稳定。与普遍表达的EIF5A1基因相反，EIF5A2在睾丸和大肠腺癌中高表达，在大脑中水平较高。两个EIF5A2 mRNA共享一个129个核苷酸的5'UTR和一个153个氨基酸的eIF5AII蛋白的编码序列，但具有两个通过差异聚腺苷酸化而形成的46和890 nt的3'UTR。该蛋白质与eIF5AI具有84％的同一性和94％的相似性。两个EIF5A基因在脊椎动物中都是保守的。我们的发现为聚胺代谢蛋白和调节剂的专门基因表达程序提供了进一步的支持，该程序可在生精过程中将聚胺稳态保持在升高的水平。版权所有2001，学术出版社。

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