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Physical and transcript map of the hereditary prostate cancer region at xq27.

机译：xq27处的遗传性前列腺癌区域的物理和转录图。

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摘要

We have recently mapped a locus for hereditary prostate cancer (termed HPCX) to the long arm of the X chromosome (Xq25-q27) through a genome-wide linkage study. Here we report the construction of an approximately 9-Mb sequence-ready bacterial clone contig map of Xq26.3-q27.3. The contig was constructed by screening BAC/PAC libraries with markers spaced at approximately 85-kb intervals. We identified overlapping clones by end-sequencing framework clones to generate 407 new sequence-tagged sites, followed by PCR verification of overlaps. Contig assembly was based on clone restriction fingerprinting and the landmark information. We identified a minimal overlap contig for genomic sequencing, which has yielded 7.7 Mb of finished sequence and 1.5 Mb of draft sequence. The transcriptional mapping effort localized 57 known and predicted genes by database searching, STS content mapping, and sequencing, followed by sequence annotation. These transcriptional units represent candidate genes for HPCX and multiple other hereditary diseases at Xq26.3-q27.3.
机译：我们最近通过全基因组连锁研究将遗传性前列腺癌（称为HPCX）的基因座定位到X染色体长臂（Xq25-q27）上。在这里，我们报告Xq26.3-q27.3的大约9-Mb序列就绪的细菌克隆重叠群图的构建。重叠群是通过用间隔大约85 kb的标记筛选BAC / PAC文库而构建的。我们通过末端测序框架克隆确定了重叠克隆，以生成407个新的序列标签位点，然后通过PCR验证重叠。重叠群装配基于克隆限制指纹图谱和地标信息。我们确定了基因组测序的最小重叠重叠群，已产生7.7 Mb的最终序列和1.5 Mb的初稿序列。转录作图工作通过数据库搜索，STS内容作图和测序，然后进行序列注释，定位了57个已知和预测的基因。这些转录单位代表Xq26.3-q27.3处HPCX和其他多种遗传性疾病的候选基因。

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