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首页> 外文期刊>European journal of mass spectrometry >Some practical aspects of the acceleration-deceleration method for ion kinetic energy focusing in matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry
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Some practical aspects of the acceleration-deceleration method for ion kinetic energy focusing in matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry

机译：基质辅助激光解吸/电离傅立叶变换离子回旋共振质谱中离子动能聚焦加速-减速方法的一些实际方面

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摘要

A new acceleration-deceleration (AD) method for reducing and focusing ion kinetic energies in matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR) mass spectrometry has been developed. The aim of the method is to equalize and then reduce the initial kinetic energy of the MALDI-produced ions, distributed over a broad energy range, by means of delayed acceleration and deceleration of the ions. The focusing of the energy results in a more efficient trapping of high mass ions and, at the same time, improves the precision of mass measurements in the ICR trap. The experimental parameters for the acceleration-deceleration ion kinetic energy focusing were predicated by means of the SIMION 3D software, incorporating both the electric and magnetic fields. The deceleration of ions both outside and inside the ICR trap was studied. The theoretical treatment shows that by applying the acceleration-deceleration method it is possible to focus the initially high kinetic energies of 100,000 Da ions into a 1 eV range and register their mass spectrum at low trapping potentials. In order to put the new method into practice, the existing FTICR mass spectrometer was equipped with a MALDI source inside the magnet. To compare the acceleration-deceleration method with other methods, the optimal mass spectrum measurement conditions for several proteomic biopolymers were studied. By means of the acceleration-deceleration method, the MALDI-FTICR mass spectrum of substance P (protonated mass 1347.736 Da) was registered at 3 * 10~(-8) mbar with mass resolution close to 70,000. A resolution of 45,000 was achieved by the gated trapping method. Bovine insulin B-chain (protonated mass 3494.65 Da) was used to compare the gated trapping method with the deceleration and acceleration-deceleration methods (which also include the gated trapping method as an essential part) at different matrix-to-analyte ratios. The acceleration-deceleration method does not reject high energy ions and has therefore inherently high sensitivity particularly at low analyte concentration. Bovine insulin (protonated mass 5732.6 Da) is so far the highest mass ion registered in our spectrometer. It was possible to measure this ion only by the acceleration-deceleration method.
机译：开发了一种新的加速-减速（AD）方法，用于减少和聚焦基质辅助激光解吸/电离傅里叶变换离子回旋共振质谱（MALDI-FTICR）质谱中的离子动能。该方法的目的是通过延迟离子的加速和减速来均衡然后减小MALDI产生的离子的初始动能，该初始动能分布在较宽的能量范围内。能量的聚集导致更有效地捕获高质量离子，同时提高了ICR阱中质量测量的精度。通过结合电场和磁场的SIMION 3D软件确定了加速-减速离子动能聚焦的实验参数。研究了ICR阱内部和外部的离子减速情况。理论处理表明，通过应用加速-减速方法，可以将最初的100,000 Da离子的高动能集中在1 eV范围内，并在低俘获电势下记录其质谱。为了将新方法付诸实践，现有的FTICR质谱仪在磁体内部配备了MALDI源。为了将加速-减速方法与其他方法进行比较，研究了几种蛋白质组生物聚合物的最佳质谱测量条件。通过加减速方法，物质P（质子化质量1347.736 Da）的MALDI-FTICR质谱在3 * 10〜（-8）mbar处记录，质量分辨率接近70,000。通过门控陷印方法获得了45,000的分辨率。牛胰岛素B链（质子化质量3494.65 Da）用于比较不同基质与分析物比率下的门控捕集方法与减速和加速-减速方法（也包括门控捕集方法作为主要部分）。加减速方法不排斥高能离子，因此固有地具有高灵敏度，尤其是在低分析物浓度下。迄今为止，牛胰岛素（质子化质量5732.6 Da）是我们光谱仪中记录的最高质量离子。可以仅通过加减速方法来测量该离子。

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