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Analysis of the promoter from an expanding mouse retrotransposon subfamily.

机译：从扩大的小鼠反转录转座子亚家族的启动子分析。

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The mouse genome contains several subfamilies of the retrotransposon L1. One subfamily, TF, contains 4000-5000 full-length members and is expanding due to retrotransposition of a large number of active elements. Here we studied the TF 5' untranslated region (UTR), which contains promoter activity required for subfamily expression. Using reporter assays, we show that promoter activity is derived from TF-specific monomer sequences and is proportional to the number of monomers in the 5' UTR. These data suggest that nearly all full-length TF elements in the mouse genome are currently competent for expression. We aligned the sequences of 53 monomers to generate a consensus TF monomer and determined that most TF elements are truncated near a potential binding site for a transcription initiation factor. We also determined that much of the sequence variation among TF monomers results from transition mutations at CpG dinucleotides, suggesting that genomic TF 5' UTRs are methylated at CpGs. Copyright 1999 Academic Press.
机译：小鼠基因组包含反转录转座子L1的几个亚家族。一个亚科TF包含4000-5000个全长成员，并且由于大量活性元件的逆转录而正在扩展。在这里，我们研究了TF 5'非翻译区（UTR），其中包含亚家族表达所需的启动子活性。使用报告基因分析，我们显示启动子活性源自TF特异性单体序列，并且与5'UTR中的单体数量成比例。这些数据表明，小鼠基因组中几乎所有全长TF元件目前都可以表达。我们比对了53个单体的序列，以生成共有的TF单体，并确定大多数TF元件在转录起始因子的潜在结合位点附近被截短。我们还确定，TF单体之间的许多序列变异是由CpG二核苷酸处的过渡突变引起的，这表明基因组TF 5'UTR在CpGs处被甲基化。版权所有1999 Academic Press。

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