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首页> 外文期刊>Genomics >Isolation, characterization, and mapping of a zinc finger gene, ZFP95, containing both a SCAN box and an alternatively spliced KRAB A domain.
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Isolation, characterization, and mapping of a zinc finger gene, ZFP95, containing both a SCAN box and an alternatively spliced KRAB A domain.

机译：锌指基因ZFP95的分离，表征和作图，它既包含SCAN框又包含可变剪接的KRABA结构域。

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摘要

A new zinc finger gene of the Kruppel family was identified by screening a human fetal cartilage cDNA library with degenerate oligonucleotides. Sequence analysis indicates that ZFP95 contains 12 highly conserved zinc finger motifs at the C-terminus and a SCAN box as well as a KRAB A domain at the N-terminus of the protein. ZFP95 represents a member of a new subclass of Kruppel zinc finger proteins containing both a SCAN box and a KRAB domain. Sequence comparison revealed that ZFP95 is the human ortholog of murine Zfp95, which is differentially expressed during spermatogenesis. We demonstrate that ZFP95 is ubiquitously expressed in adult and fetal tissues with the strongest expression in testis. Two transcripts, 4. 2 and 4.6 kb, were detected in all tissues tested. In testis, a third transcript of 3.8 kb was present. RT-PCR analysis confirmed alternative splicing for the KRAB A domain and an upstream exon leading to three transcripts of ZFP95 with and without this transcriptional repressor domain. Finally, we show that ZFP95 maps on human chromosome 7q22 between the markers D7S651 and WI-5853. Copyright 1999 Academic Press.
机译：通过用简并的寡核苷酸筛选人胎儿软骨cDNA文库，鉴定出Kruppel家族的新锌指基因。序列分析表明，ZFP95在蛋白的C端和SCAN盒中含有12个高度保守的锌指基序，在蛋白的N端含有KRAB A结构域。 ZFP95代表同时包含SCAN框和KRAB结构域的Kruppel锌指蛋白新亚类的成员。序列比较显示ZFP95是鼠Zfp95的人类直系同源基因，在精子发生过程中差异表达。我们证明ZFP95在成人和胎儿组织中普遍存在，在睾丸中的表达最强。在所有测试的组织中检测到两个转录本：4。2和4.6 kb。在睾丸中，存在3.8kb的第三转录物。 RT-PCR分析证实了KRABA结构域和上游外显子的选择性剪接，导致带有和不带有该转录抑制子结构域的ZFP95的三个转录物。最后，我们显示ZFP95在人染色体7q22上标记D7S651和WI-5853之间。版权所有1999 Academic Press。

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