...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >Elucidation of the minimal sequence required to imprint H19 transgenes.
【24h】

Elucidation of the minimal sequence required to imprint H19 transgenes.

机译：阐明印记H19转基因所需的最小序列。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The imprinted mouse H19 gene exhibits maternal allele-specific expression and paternal allele-specific hypermethylation. We previously demonstrated that a 14-kb H19 minitransgene possessing 5' differentially methylated sequence recapitulates the endogenous H19 imprinting pattern when present as high-copy arrays. To investigate the minimal sequences that are sufficient for H19 transgene imprinting, we have tested new transgenes in mice. While transgenes harboring limited or no 3' H19 sequence indicate that multiple elements within the 8-kb 3' fragment are required for appropriate imprinting, transgenes incorporating 1.7 kb of additional 5' sequence mimic the endogenous H19 pattern, including proper imprinting of low-copy arrays. One of these imprinted lines had a single 15.7-kb transgene integrant. This is the smallest H19 transgene identified thus far to display imprinting properties characteristic of the endogenous gene, suggesting that all cis-acting elements required for H19 imprinting in endodermal tissues reside within the 15.7-kb transgenic sequence. Copyright 2001 Academic Press.
机译：印迹的小鼠H19基因表现出母本等位基因特异性表达和父本等位基因特异性高甲基化。我们以前证明具有5'差异甲基化序列的14 kb H19小转基因在以高拷贝阵列形式存在时概括了内源性H19印迹模式。为了研究足以完成H19转基因印迹的最小序列，我们已经在小鼠中测试了新的转基因。带有有限的3'H19序列或没有3'H19序列的转基因表明适当的印迹需要8kb 3'片段中的多个元件，而掺入1.7 kb的其他5'序列的转基因模仿内源性H19模式，包括低拷贝的正确印迹数组。这些印迹品系之一具有单个15.7-kb转基因整合体。这是迄今为止鉴定出的最小的H19转基因，显示出内源基因的印记特性，这表明内皮组织中H19印记所需的所有顺式作用元件均位于15.7-kb转基因序列内。版权所有2001，学术出版社。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号