Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig.

Zhao SH; Recknor J; Lunney JK; Nettleton D; Kuhar D; Orley S; Tuggle CK

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Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig.

机译：验证第一代长寡核苷酸微阵列在猪中的转录谱。

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A first-generation porcine oligonucleotide set, representing 13,297 cDNAs and ESTs, has been designed by Qiagen-Operon for transcriptional profiling. To validate this set, microarrays containing each 70-mer oligonucleotide, referred to as the Qiagen-NRSP8 array, were hybridized with targets from porcine adult liver, lung, muscle, or small intestine. Transcriptome analyses showed that 11,328 of the oligonucleotides demonstrated expression in at least one tissue. Statistical analyses revealed that 1810 genes showed differential expression among tissues (Bonferroni adjusted p < 0.05). Biological pathways identified by DAVID/EASE analysis using a list of 423 tissue-selective genes matched archetypal pathways in the corresponding human or mouse tissue. Real-time quantitative PCR confirmed expression patterns for 9 of 11 genes tested. Our results demonstrate that this first-generation porcine oligonucleotide array is informative and the specificity is high. This is essential validation for investigators using the Qiagen-NRSP8 array for porcine functional genomics and for using the pig in modeling important physiological problems.

机译：Qiagen-Operon设计了代表13297个cDNA和EST的第一代猪寡核苷酸，用于转录谱分析。为了验证这一组，将包含每个70-mer寡核苷酸的微阵列（称为Qiagen-NRSP8阵列）与来自猪成年肝，肺，肌肉或小肠的靶标杂交。转录组分析显示11,328个寡核苷酸在至少一种组织中表现出表达。统计分析表明，有1810个基因在组织之间显示差异表达（Bonferroni调整后的p <0.05）。通过DAVID / EASE分析，使用423种组织选择基因列表确定的生物途径与相应的人或小鼠组织中的原型途径相匹配。实时定量PCR证实了所测试的11个基因中有9个的表达模式。我们的结果表明，这种第一代猪寡核苷酸阵列具有丰富的信息，而且特异性很高。对于使用Qiagen-NRSP8阵列进行猪功能基因组分析的研究人员以及使用猪对重要的生理问题进行建模，这是至关重要的验证。

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《Genomics》 |2005年第5期|共8页
作者
Zhao SH; Recknor J; Lunney JK; Nettleton D; Kuhar D; Orley S; Tuggle CK;
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正文语种 eng
中图分类基础医学;
关键词
Swine; Oligonucleotides; Tissues; functional genomics; Expressed Sequence Tags; 猪; 寡核苷酸类; 组织; 表达序列标记;

机译：Swine;Oligonucleotides;Tissues;functional genomics;Expressed Sequence Tags;猪;寡核苷酸类;组织;表达序列标记;

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1. Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig. [J] . Zhao SH, Recknor J, Lunney JK, Genomics . 2005,第5期

机译：验证第一代长寡核苷酸微阵列在猪中的转录谱。
2. Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays [J] . Yulei Wang, Catalin Barbacioru, Fiona Hyland, BMC Genomics . 2006,第1期

机译：大规模实时PCR验证从两个商业长寡核苷酸微阵列的基因表达测量
3. Design and validation of a partial-genome microarray for transcriptional profiling of the Bradyrhizobium japonicum symbiotic gene region [J] . Hauser F, Lindemann A, Vuilleumier S, Molecular biology reports . 2006,第1期

机译：设计和验证部分基因组微阵列用于日本根瘤菌共生基因区域的转录谱分析
4. Cloud-Accelerated Data-Mining for Putative Heteromeric Transcription Factors and Target Genes Using Microarray Gene Expression Profiles [C] . Edward A. Salinas, Amitava Karmaker International Conference on Bioinformatics and Computational Biology . 2013

机译：使用微阵列基因表达谱的推定异致转录因子和靶基因的云加速数据挖掘
5. DNA microarray analysis and statistical validation for expression profiling in Escherichia coli. [D] . Oh, Min-Kyu. 2001

机译：DNA微阵列分析和统计验证，用于在大肠杆菌中进行表达谱分析。
6. Construction and validation of a first-generation Bordetella bronchiseptica long-oligonucleotide microarray by transcriptional profiling the Bvg regulon [O] . Tracy L Nicholson 2007

机译：通过对Bvg调节子进行转录分析来构建和验证第一代支气管败血博德特氏菌长寡核苷酸微阵列
7. Construction and validation of a first-generation Bordetella bronchiseptica long-oligonucleotide microarray by transcriptional profiling the Bvg regulon [O] . Nicholson Tracy L 2007

机译：通过转录分析Bvg调节子构建第一代支气管败血博德特氏菌长寡核苷酸微阵列并进行验证
8. Use of DNA Microarrays to Identify Diagnostic Signature Transcriptional Profiles for Host Responses to Infectious Agents [R] . Ellner, J. J. , Connell, N. D. , Gallagher, G. , 2005

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Validation of a first-generation long-oligonucleotide microarray for transcriptional profiling in the pig.

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