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首页> 外文期刊>Genomics >Btrim: a fast, lightweight adapter and quality trimming program for next-generation sequencing technologies.
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Btrim: a fast, lightweight adapter and quality trimming program for next-generation sequencing technologies.

机译:Btrim:一种快速,轻便的适配器和用于下一代测序技术的高质量修整程序。

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摘要

Btrim is a fast and lightweight software to trim adapters and low quality regions in reads from ultra high-throughput next-generation sequencing machines. It also can reliably identify barcodes and assign the reads to the original samples. Based on a modified Myers's bit-vector dynamic programming algorithm, Btrim can handle indels in adapters and barcodes. It removes low quality regions and trims off adapters at both or either end of the reads. A typical trimming of 30M reads with two sets of adapter pairs can be done in about a minute with a small memory footprint. Btrim is a versatile stand-alone tool that can be used as the first step in virtually all next-generation sequence analysis pipelines. The program is available at http://graphics.med.yale.edu/trim/.
机译:Btrim是一款快速轻巧的软件,可在超高通量下一代测序仪的读取中修整适配器和低质量区域。它还可以可靠地识别条形码,并将读数分配给原始样品。基于改进的Myers位矢量动态编程算法,Btrim可以处理适配器和条形码中的插入缺失。它会删除低质量的区域,并在读取的两端或两端修剪适配器。使用两组适配器对进行的典型30M读取修整可以在大约一分钟内完成,并且占用的内存很小。 Btrim是一种多功能的独立工具,可以用作几乎所有下一代序列分析管道中的第一步。该程序可从http://graphics.med.yale.edu/trim/获得。

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