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首页> 外文期刊>Genomics >Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family.
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Molecular cloning and characterization of NEU4, the fourth member of the human sialidase gene family.

机译：人唾液酸酶基因家族的第四个成员NEU4的分子克隆和表征。

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摘要

Several mammalian sialidases have been cloned so far and here we describe the identification and expression of a new member of the human sialidase gene family. The NEU4 gene, identified by searching sequence databases for entries showing homologies to the human cytosolic sialidase NEU2, maps in 2q37 and encodes a 484-residue protein. The polypeptide contains all the typical sialidase amino acid motifs and, apart from an amino acid stretch that appears unique among mammalian sialidases, shows a high degree of homology for NEU2 and the plasma membrane-associated (NEU3) sialidases. RNA dot-blot analysis showed a low but wide expression pattern, with the highest level in liver. Transient transfection in COS7 cells allowed the detection of a sialidase activity toward the artificial substrate 4MU-NeuAc in the acidic range of pH. Immunofluorescence staining and Western blot analysis demonstrated the association of NEU4 with the inner cell membranes.
机译：到目前为止已经克隆了几种哺乳动物唾液酸酶，在这里我们描述了人类唾液酸酶基因家族新成员的鉴定和表达。 NEU4基因通过搜索序列数据库来确定与人胞质唾液酸酶NEU2同源的条目，可在2q37中定位并编码484个残基蛋白。该多肽包含所有典型的唾液酸酶氨基酸基序，并且除了在哺乳动物唾液酸酶中似乎独特的氨基酸序列外，还显示出与NEU2和质膜相关（NEU3）唾液酸酶高度同源。 RNA点印迹分析显示出较低但较宽的表达模式，肝脏中的表达水平最高。 COS7细胞中的瞬时转染可以检测到在酸性pH范围内针对人工底物4MU-NeuAc的唾液酸酶活性。免疫荧光染色和Western印迹分析表明NEU4与内细胞膜的关联。

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