首页> 外文期刊>Genomics >The first radiation hybrid map of a perch-like fish: the gilthead seabream (Sparus aurata L).
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The first radiation hybrid map of a perch-like fish: the gilthead seabream (Sparus aurata L).

机译:鲈鱼状鱼类的第一个辐射混合图:金头鲷(Sparus aurata L)。

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Among Teleosts, Perciformes are the largest order of fishes and include numerous species of commercial importance. Perciformes also comprise species of primary interest for evolutionary studies and analysis of the sex determination systems and sex chromosome plasticity. Unfortunately, genomics tools and resources for Perciformes remain to be developed. Here, we report the production of a seabream whole-genome radiation hybrid (RH) panel in which quality was ascertained by the construction of a 2-Mb-resolution RH map. The map encompasses 440 markers (288 microsatellites, 82 gene-based markers, and 70 STS) suitable for linkage analysis and comparative mapping studies. Achievement of a RH panel and a whole-genome RH map should contribute to establishing seabream as a fish model among the Perciformes and should be of importance in aquaculture for marker-assisted selection, improvement of growth performance, and disease management. Development of RH maps in a cost-effective manner for other fishes with the described methodology will offer a powerful approach in aquaculture and will provide extended capabilities for comparing vertebrate genome evolution.
机译:在硬骨鱼类中,鲈形目是鱼类的最大订单,并且包括许多具有商业重要性的物种。蛇形目还包括主要用于进化研究和性别决定系统和性染色体可塑性分析的物种。不幸的是,Perciformes的基因组学工具和资源仍有待开发。在这里,我们报告了鲷鱼全基因组辐射杂交(RH)面板的生产,该面板的质量是通过构建2 Mb分辨率的RH图来确定的。该图谱包含适合进行连锁分析和比较作图研究的440个标记(288个微卫星,82个基于基因的标记和70个STS)。相对湿度小组和全基因组相对湿度图谱的实现应有助于在鲷科动物中建立鲷鱼的鱼类模型,并在水产养殖中对标记辅助选择,改善生长性能和疾病管理具有重要意义。利用所描述的方法以经济有效的方式为其他鱼类开发RH图谱,将为水产养殖提供有力的方法,并为比较脊椎动物基因组进化提供扩展的能力。

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