...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >High-density mapping and comparative analysis of agronomically important traits on wheat chromosome 3A.
【24h】

High-density mapping and comparative analysis of agronomically important traits on wheat chromosome 3A.

机译：小麦3A染色体上重要农艺性状的高密度作图和比较分析。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bread wheat chromosome 3A has been shown to contain genes/QTLs controlling grain yield and other agronomic traits. The objectives of this study were to generate high-density physical and genetic-linkage maps of wheat homoeologous group 3 chromosomes and reveal the physical locations of genes/QTLs controlling yield and its component traits, as well as agronomic traits, to obtain a precise estimate of recombination for the corresponding regions and to enrich the QTL-containing regions with markers. Physical mapping was accomplished by 179 DNA markers mostly representing expressed genes using 41 single-break deletion lines. Polymorphism survey of cultivars Cheyenne (CNN) and Wichita (WI), and a substitution line of CNN carrying chromosome 3A from WI [CNN(WI3A)], with 142 RFLP probes and 55 SSR markers revealed that the extent of polymorphism is different among various group 3 chromosomal regions as well as among the homoeologs. A genetic-linkage map for chromosome 3A was developed by mapping 17 QTLs for seven agronomic traits relative to 26 RFLP and 15 SSR chromosome 3A-specific markers on 95 single-chromosome recombinant inbred lines. Comparison of the physical maps with the 3A genetic-linkage map localized the QTLs to gene-containing regions and accounted for only about 36% of the chromosome. Two chromosomal regions containing 9 of the 17 QTLs encompassed less than 10% of chromosome 3A but accounted for almost all of the arm recombination. To identify rice chromosomal regions corresponding to the particular QTL-containing wheat regions, 650 physically mapped wheat group 3 sequences were compared with rice genomic sequences. At an E value of E < or = 10(-5), 82% of the wheat group 3 sequences identified rice homologs, of which 54% were on rice chromosome 1. The rice chromosome 1 region collinear with the two wheat regions that contained 9 QTLs was about 6.5 Mb.
机译：面包小麦3A染色体已显示含有控制谷物产量和其他农艺性状的基因/ QTL。这项研究的目的是生成小麦同源3组染色体的高密度物理和遗传连锁图谱，并揭示控制产量和其组成性状以及农艺性状的基因/ QTL的物理位置，以获得精确的估计值。重组相应区域并用标记富集含有QTL的区域。物理作图是通过使用41条单断裂删除系由179个主要代表表达基因的DNA标记完成的。品种夏安（CNN）和威奇托（WI）的多态性调查，以及来自WI [CNN（WI3A）]的带有3A染色体的CNN替代品系，用142个RFLP探针和55个SSR标记表明，不同基因的多态性程度不同第3组染色体区域以及同系物之间。通过在95个单染色体重组自交系上绘制相对于26个RFLP和15个SSR染色体3A特异性标记的7个农艺性状的17个QTL，绘制了3A染色体的遗传连锁图。物理图谱与3A遗传连锁图谱的比较将QTL定位于含有基因的区域，仅占染色体的36％。包含17个QTL中的9个的两个染色体区域包含的染色体3A不到10％，但几乎占了臂重组的全部。为了鉴定对应于特定含QTL小麦区域的水稻染色体区域，将650个物理定位的小麦第3组序列与水稻基因组序列进行了比较。在E值E <或= 10（-5）时，小麦第3组序列的82％鉴定出了水稻同源物，其中54％在水稻1号染色体上。水稻1号染色体区域与包含该序列的两个小麦区域共线9个QTL约为6.5 Mb。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号