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首页> 外文期刊>Genomics >Conserved tissue expression signatures of intronic noncoding RNAs transcribed from human and mouse loci.
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Conserved tissue expression signatures of intronic noncoding RNAs transcribed from human and mouse loci.

机译：从人和小鼠基因座转录的内含子非编码RNA的保守组织表达特征。

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It has been postulated that noncoding RNAs (ncRNAs) are involved in the posttranscriptional control of gene expression, and may have contributed to the emergence of the complex attributes observed in mammalians. We show here that the complement of ncRNAs expressed from intronic regions of the human and mouse genomes comprises at least 78,147 and 39,660 transcriptional units, respectively. To identify conserved intronic sequences expressed in both humans and mice, we used custom-designed human cDNA microarrays to separately interrogate RNA from mouse and human liver, kidney, and prostate tissues. An overlapping tissue expression signature was detected for both species, comprising 198 transcripts; among these, 22 RNAs map to intronic regions with evidence of evolutionary conservation in humans and mice. Transcription of selected human-mouse intronic ncRNAs was confirmed using strand-specific RT-PCR. Altogether, these results support an evolutionarily conserved role of intronic ncRNAs in human and mouse, which are likely to be involved in the fine tuning of gene expression regulation in different mammalian tissues.
机译：据推测，非编码RNA（ncRNA）参与基因表达的转录后控制，并且可能有助于哺乳动物中观察到的复杂属性的出现。我们在这里显示，从人和小鼠基因组的内含子区域表达的ncRNA的互补序列分别包含至少78,147和39,660个转录单位。为了鉴定在人类和小鼠中表达的保守内含子序列，我们使用了定制设计的人类cDNA微阵列来分别询问小鼠和人类肝脏，肾脏和前列腺组织中的RNA。两种物种均检测到重叠的组织表达特征，包括198个转录本;其中，有22个RNA定位到内含子区域，具有人类和小鼠进化保守的证据。使用链特异性RT-PCR证实了选定的人类小鼠内含子ncRNA的转录。总而言之，这些结果支持了人类和小鼠中内含子ncRNA在进化上的保守作用，它们可能参与了不同哺乳动物组织中基因表达调控的微调。

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