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首页> 外文期刊>Genomics >DNA methylation in the IGF2 intragenic DMR is re-established in a sex-specific manner in bovine blastocysts after somatic cloning.
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DNA methylation in the IGF2 intragenic DMR is re-established in a sex-specific manner in bovine blastocysts after somatic cloning.

机译：体细胞克隆后，IGF2基因内DMR中的DNA甲基化以性别特异性方式在牛胚泡中重新建立。

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摘要

The recent identification of an intragenic differentially methylated region (DMR) within the last exon of the bovine Insulin-like growth factor 2 (IGF2) gene provides a diagnostic tool for in-depth investigation of bovine imprinting and regulatory mechanisms which are active during embryo development. Here, we used bisulfite sequencing to compare sex-specific DNA methylation patterns within this DMR in bovine blastocysts produced in vivo, by in vitro fertilization and culture, SCNT, androgenesis or parthenogenesis. In in vivo derived embryos, DNA methylation was removed from this intragenic DMR after fertilization, but partially replaced by the time the embryo reached the blastocyst stage. Among embryos developing in vivo, the level of DNA methylation was significantly lower in female than in male blastocysts. This sexual dimorphism was also found between parthenogenetic and androgenetic embryos, and followed the donor cell sex in SCNT derived blastocysts and is evidence for correct methylation reprogramming in SCNT embryos.
机译：最近鉴定出牛胰岛素样生长因子2（IGF2）基因最后一个外显子中的基因内差异甲基化区域（DMR），为深入研究在胚胎发育过程中活跃的牛印迹和调控机制提供了一种诊断工具。在这里，我们使用亚硫酸氢盐测序来比较通过体外受精和培养，SCNT，雄激素生成或孤雌生殖而在体内产生的牛胚泡中DMR内的性别特异性DNA甲基化模式。在体内衍生的胚胎中，受精后从该基因内DMR中去除了DNA甲基化，但在胚胎到达胚泡期时被部分替代。在体内发育的胚胎中，女性的DNA甲基化水平明显低于男性的胚泡。在单性生殖和雄激素的胚胎之间也发现了这种有性二态性，并遵循了SCNT衍生的胚泡中的供体细胞性别，这是SCNT胚胎中正确甲基化重编程的证据。

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