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首页> 外文期刊>Genomics >Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression.
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Selection and mapping of replication origins from a 500-kb region of the human X chromosome and their relationship to gene expression.

机译：从人类X染色体的500-kb区域复制起点的选择和作图及其与基因表达的关系。

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摘要

In higher eukaryotes the mechanism controlling initiation of DNA replication remains largely unknown. New technologies are needed to shed light on how DNA replication initiates along the genome in specific regions. To identify the human DNA sequence requirements for initiation of replication, we developed a new method that allows selection of replication origins starting from large genomic regions of human DNA. We repeatedly isolated 15 new putative replication origins (PROs) from a human DNA region of 500 kb in which 17 genes have previously been characterized. Fine-mapping of these PROs showed that DNA replication can initiate at many specific points along actively transcribed DNA in the cell lines used for our selection. In conclusion, in this paper we describe a new method to identify PROs that suggests that the availability of initiation sites is dependent on the transcriptional state of the DNA. Copyright 1999 Academic Press.
机译：在高级真核生物中，控制DNA复制起始的机制仍然是未知的。需要新技术来阐明DNA复制如何沿着特定区域的基因组启动。为了确定复制起始所需的人类DNA序列要求，我们开发了一种新方法，该方法允许从人类DNA的大基因组区域开始选择复制起点。我们从500 kb的人类DNA区域（其中先前已鉴定了17个基因）中反复分离出15个新的假定复制起点（PROs）。这些PRO的精细映射显示DNA复制可以在我们选择的细胞系中沿着活跃转录的DNA的许多特定点启动。总之，在本文中，我们描述了一种鉴定PRO的新方法，该方法表明起始位点的可用性取决于DNA的转录状态。版权所有1999 Academic Press。

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