• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >A functional in vivo screen for regulators of tumor progression identifies HOXB2 as a regulator of tumor growth in breast cancer.
【24h】

A functional in vivo screen for regulators of tumor progression identifies HOXB2 as a regulator of tumor growth in breast cancer.

机译：对肿瘤进程调节剂的功能性体内筛选将HOXB2鉴定为乳腺癌中肿瘤生长的调节剂。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microarray profiling in breast cancer patients has identified genes correlated with prognosis whose functions are unknown. The purpose of this study was to develop an in vivo assay for functionally screening regulators of tumor progression using a mouse model. Transductant shRNA cell lines were made in the MDA-MB-231 breast cancer line. A pooled population of 25 transductants was injected into the mammary fat pads and tail veins of mice to evaluate tumor growth, and experimental metastasis. The proportions of transductants were evaluated in the tumor and metastases using barcodes specific to each shRNA transductant. We characterized the homeobox 2 transcription factor as a negative regulator, decreasing tumor growth in MDA-MB-231, T47D, and MTLn3 mammary adenocarcinoma cell lines. Homeobox genes have been correlated with cancer patient prognosis and tumorigenesis. Here we use a novel in vivo shRNA screen to identify a new role for a homeobox gene in human mammary adenocarcinoma.
机译：乳腺癌患者的微阵列分析已鉴定出与功能未知的预后相关的基因。这项研究的目的是开发一种体内实验，用于使用小鼠模型功能性筛选肿瘤进展调节剂。在MDA-MB-231乳腺癌细胞系中制备了转导shRNA细胞系。将汇集的25种转导子群体注入小鼠的乳腺脂肪垫和尾静脉中，以评估肿瘤的生长和实验转移。使用每种shRNA转导子特异的条形码评估肿瘤和转移中转导子的比例。我们将同源盒2转录因子表征为负调节剂，从而降低了MDA-MB-231，T47D和MTLn3乳腺腺癌细胞系中的肿瘤生长。同源盒基因已经与癌症患者的预后和肿瘤发生相关。在这里，我们使用一种新颖的体内shRNA筛查方法来鉴定同源异型盒基因在人乳腺腺癌中的新作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号