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Quality indicators increase the reliability of microarray data.

机译：质量指标提高了微阵列数据的可靠性。

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Large-scale gene expression profiling with DNA microarrays opens new dimensions to molecular biology but still lacks the overall precision of traditional low-scale techniques. We developed a novel strategy of data processing linking search stringency to quality indicators for efficient detection of low-level, regulated genes. Using retinoid-induced differentiation of NB-4 promyelocytic cells, the variation of expression profiles between biological duplicates was studied and compared with the changes induced by all-trans retinoic acid (atRA) treatment. An analysis of 4320 genes showed that retinoic acid has mainly geneactivating function in NB-4 cells. Treatment with atRA for 18 hours induced metabolic genes that may be associated with cell differentiation and signaling factors triggering later events leading to apoptosis; cytokine genes were among the highest stimulated by atRA. Notably, we identified a regulatory loop inhibiting MYC action: as MYC was downregulated, a cognate repressor of MYC was upregulated.
机译：DNA微阵列的大规模基因表达谱分析为分子生物学开辟了新的领域，但仍缺乏传统低规模技术的整体精度。我们开发了一种新的数据处理策略，将搜索严格性与质量指标联系起来，以有效检测低水平，受调控的基因。使用类维生素A诱导的NB-4早幼粒细胞分化，研究了生物学复制品之间的表达谱变化，并将其与全反式维甲酸（atRA）处理引起的变化进行了比较。对4320个基因的分析表明，视黄酸在NB-4细胞中主要具有基因激活功能。用atRA治疗18小时后，诱导的代谢基因可能与细胞分化和信号转导因子相关，从而触发后来导致细胞凋亡的事件;细胞因子基因是atRA刺激的最高基因之一。值得注意的是，我们发现了一个抑制MYC作用的调节环：当MYC被下调时，MYC的同源阻遏物被上调。

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