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首页> 外文期刊>Genomics >The prepro vasoactive intestinal contractor (VIC)/endothelin-2 gene (EDN2): structure, evolution, production, and embryonic expression.
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The prepro vasoactive intestinal contractor (VIC)/endothelin-2 gene (EDN2): structure, evolution, production, and embryonic expression.

机译：前血管活性肠收缩蛋白（VIC）/内皮素2基因（EDN2）：结构，进化，产生和胚胎表达。

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摘要

Murine vasoactive intestinal contractor (VIC) and its human analog endothelin-2 (ET2) are potent vasoactive hormones composed of 21 amino acids. To study the structural characteristics of the VIC/ET2 gene (HGMW-approved symbol EDN2), we isolated the full length of the mouse VIC gene. Sequence analysis indicates that a biologically active mature VIC peptide is produced from a 175-residue precursor protein; preproVIC (PPVIC). Several remarkable similarities of the PPVIC gene to the human preproendothelin-1 gene strongly suggest that the two genes have arisen from a common progenitor by gene duplication. Transfection of ACHN adenocarcinoma cells with the cDNA resulted in the production of VIC peptide. VIC production was increased by the deletion of the 3'-untranslated region, which contains an AU-rich mRNA destabilizing sequence. Increased PPVIC gene expression during the late embryonic stage suggests an important function in development. This study provides the basis for disruption and regulation analysis of the gene, which may lead to a better understanding of VIC/ET2's physiological significance. Copyright 2000 Academic Press.
机译：鼠血管活性肠收缩剂（VIC）及其人类类似物内皮素2（ET2）是有效的血管活性激素，由21个氨基酸组成。为了研究VIC / ET2基因（HGMW批准的符号EDN2）的结构特征，我们分离了小鼠VIC基因的全长。序列分析表明，具有生物活性的成熟VIC肽是由175个残基的前体蛋白产生的。 preproVIC（PPVIC）。 PPVIC基因与人preproendothelin-1基因的几个显着相似之处强烈表明，这两个基因是通过基因复制来自共同祖细胞的。用该cDNA转染ACHN腺癌细胞导致产生VIC肽。通过缺失3'非翻译区（包含富含AU的mRNA不稳定序列）增加了VIC的产生。在胚胎后期，PPVIC基因表达的增加表明其在发育中具有重要作用。这项研究为基因的破坏和调控分析提供了基础，这可能有助于更好地了解VIC / ET2的生理学意义。版权所有2000学术出版社。

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