• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse.
【24h】

Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse.

机译：开发用于实验室小鼠遗传监测的SNP基因分型面板。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have developed a genotyping system for detecting genetic contamination in the laboratory mouse based on assaying single-nucleotide polymorphism (SNP) markers positioned on all autosomes and the X chromosome. This system provides a fast, reliable, and cost-effective way for genetic monitoring, while maintaining a very high degree of confidence. We describe the allelic distribution of 235 SNPs in 48 mouse strains, thereby creating a database of polymorphisms useful for genotyping purposes. The SNP markers used in this study were chosen from publicly available SNP databases. Four genotyping methods were evaluated, and dynamic two-tube allele-specific PCR assays were developed for each marker and tested on a set of 48 inbred mouse strains. The minimal number of assays sufficient to distinguish groups consisting of different numbers of mouse strains was estimated, and a panel of 28 SNPs sufficient to distinguish virtually all of the inbred strains tested was selected. Amplifluor SNP detection assays weredeveloped for these markers and tested on an extended list of 96 strains. This panel was used as a genetic quality control approach to monitor the genotypes of nearly 300 inbred, wild-derived, congenic, consomic, and recombinant inbred strains maintained at The Jackson Laboratory. We have concluded that this marker panel is sufficient for genetic contamination monitoring in colonies containing a large number of genetically diverse mouse strains and that reduced versions of the panel could be implemented in facilities housing a lower number of strains.
机译：我们已经开发了一种基因型分型系统，用于通过检测位于所有常染色体和X染色体上的单核苷酸多态性（SNP）标记来检测实验室小鼠的遗传污染。该系统提供了一种快速，可靠且经济高效的遗传监测方法，同时保持了很高的可信度。我们描述了48个小鼠品系中235个SNP的等位基因分布，从而创建了可用于基因分型目的的多态性数据库。本研究中使用的SNP标记选自可公开获得的SNP数据库。评估了四种基因分型方法，并为每种标记物开发了动态的两管等位基因特异性PCR检测方法，并在一组48个近交小鼠品系上进行了测试。估计足以区分由不同数量的小鼠品系组成的组的最少分析数量，并选择了一组足以区分几乎所有测试的近交品系的28个SNP。针对这些标记物开发了Amplifluor SNP检测方法，并在96个菌株的扩展列表上进行了测试。该小组被用作遗传质量控制方法，以监测杰克逊实验室保存的近300种近交，野生来源，同基因，纯合和重组近交菌株的基因型。我们得出的结论是，该标记物组足以在包含大量遗传上不同的小鼠品系的菌落中进行遗传污染监测，并且可以在容纳较少数量品系的设施中实施简化版的标本。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号