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首页> 外文期刊>Genomics >Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region.
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Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region.

机译:利用斑马雀科BAC库确定禽类雄激素受体基因组区域的结构。

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摘要

The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.
机译:斑胸草雀(Taeniopygia guttata)是研究行为,神经科学,禽类生物学和进化的重要模型生物。为了支持其基因组研究,我们使用了来自女性肝脏的DNA构建了BAC文库(TG__Ba)。 BAC文库由147,456个克隆组成,其中98%包含平均大小为134 kb的插入片段,代表15.5个单倍体基因组当量。通过对整个BAC进行测序,确定了全长雄激素受体开放阅读框,这是禽类中的第一个阅读框。 BAC末端序列和整个BAC序列与鸡基因组吃水序列的比较表明,斑马雀科和鸡基因组之间具有高度保守的同义性。

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