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首页> 外文期刊>Genomics >The expression of prophenoloxidase mRNA in red swamp crayfish, Procambarus clarkii, when it was challenged
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The expression of prophenoloxidase mRNA in red swamp crayfish, Procambarus clarkii, when it was challenged

机译：受到挑战的红色沼泽小龙虾Procambarus clarkii中的酚氧化酶原mRNA表达

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摘要

The expression of the prophenoloxidase (proPO) gene was investigated in nine tissues of red swamp crayfish Procambarus clarkii, by real-time PCR after challenges by CpG oligodeoxynucleotide (ODN), Aeromonas hydrophila and white spot syndrome virus (WSSV). The results can be summarized as follows: (i) the expression level of the proPO gene in haemocytes was highest among nine studied tissues before the challenge; (ii) the expression of proPO increased in all studied tissues after stimulation by CpG ODN and WSSV, and also increased in all tissues, except the ovary, after the A. hydrophila challenge; (iii) the whole expression profiles were different, suggesting that different immune mechanisms may exist for crayfish that are resistant to WSSV and A. hydrophila, although the expression in haemocytes was similar before and after the WSSV and A. hydrophila challenges.
机译：在CpG寡脱氧核苷酸（ODN），嗜水气单胞菌和白斑综合症病毒（WSSV）攻击后，通过实时PCR研究了红沼泽小龙虾克氏原螯虾的9个组织中前酚氧化酶（proPO）基因的表达。结果可总结如下：（i）在攻击之前的九个研究组织中，血红细胞中proPO基因的表达水平最高; （ii）在亲水性链球菌攻击后，CpG ODN和WSSV刺激后，所有研究的组织中proPO的表达均增加，除卵巢外，所有组织中proPO的表达均增加; （iii）整个表达谱是不同的，这表明小龙虾对WSSV和A. hydrophila有抗性，但在血细胞中的表达在WSSV和A. hydrophila攻击前后相似，因此可能存在不同的免疫机制。

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