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首页> 外文期刊>Genomics >Profiling the metatranscriptome of the protistan community in Coptotermes formosanus with emphasis on the lignocellulolytic system
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Profiling the metatranscriptome of the protistan community in Coptotermes formosanus with emphasis on the lignocellulolytic system

机译：剖析台湾白蚁（Coptotermes formosanus）原生动物群落的转录组，侧重于木质纤维素分解系统

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摘要

The symbiotic protists in the hindgut of lower termites are critical for lignocellulose decomposition. Due to the unculturability of these protists, information on lignocellulases and their abundance within the gut is unavailable. The advent of high-throughput sequencing technologies enables an investigation of the gene expression profile in this community without culturing these organisms. Here, we carried out 454 pyrosequencing to profile the metatranscriptome of the protistan community in Coptotermes formosanus. In total, 223,477 reads were obtained by sequencing the enriched protistan mRNA. Phagocytosis and cytoskeletal homeostasis pathways were highly represented in the metatranscriptome. Among the metabolic pathways, starch and sucrose metabolism were dominant. A detailed analysis combining Pfam and KEGG annotation identified 118 glycosyl hydrolases belonging to 18 different glycosyl hydrolase families (GHFs). Subsequently, a novel GHF10 endo-1,4-beta-xylanase was functionally characterized to complement our understanding of the protistan hemicellulases.
机译：下白蚁后肠中的共生生物对于木质纤维素的分解至关重要。由于这些原生生物不可培养，因此无法获得有关木质纤维素酶及其在肠道内的丰度的信息。高通量测序技术的出现使得能够在不培养这些生物的情况下研究该群体中的基因表达谱。在这里，我们进行了454次焦磷酸测序，以分析台湾白蚁中protistan群落的转录组。通过对富集的前列腺素mRNA进行测序，总共获得了223,477个读数。在转录组中高度代表了吞噬作用和细胞骨架稳态途径。在代谢途径中，淀粉和蔗糖代谢占主导。结合Pfam和KEGG注释进行的详细分析确定了118个糖基水解酶，它们分别属于18个不同的糖基水解酶家族（GHF）。随后，一种新型GHF10内-1,4-β-木聚糖酶的功能得到了补充，以补充我们对前列腺素半纤维素酶的理解。

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