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Synteny Comparison between Apes and Human Using Fine-Mapping of the Genome.

机译:使用基因组精细映射在猿和人类之间进行同义比较。

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摘要

Comparing the genomes of the great apes and human should provide novel information concerning the origins of humankind. Relative to the great apes, the human karyotype has one fewer chromosome pair, as human chromosome 2 derived from the telomeric fusion of two ancestral primate chromosomes. To identify the genomic rearrangements that accompanied human speciation, we initiated a comparative study between human, chimpanzee, and gorilla. Using the HAPPY mapping method, an acellular adaptation of the radiation hybrid method, we mapped a few hundred markers on the human, chimpanzee, and gorilla genomes. This allowed us to identify several chromosome rearrangements, in particular a pericentric inversion and a translocation. We precisely localized the synteny breakpoint that led to the formation of human chromosome 2. This breakpoint was confirmed by FISH mapping.
机译:比较大猿和人类的基因组应该提供有关人类起源的新颖信息。相对于大猿,人类染色体核型少一对,因为人类染色体2来自两个祖传灵长类染色体的端粒融合。为了确定伴随人类物种形成的基因组重排,我们启动了人类,黑猩猩和大猩猩之间的比较研究。使用HAPPY定位方法(一种放射杂交方法的无细胞适应方法),我们在人类,黑猩猩和大猩猩基因组上定位了数百个标记。这使我们能够确定几种染色体重排,特别是周向倒置和易位。我们精确定位了导致人类2号染色体形成的协同断裂点。此断裂点已通过FISH映射得到证实。

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