...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Genomics >ChIP-chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation experiments.
【24h】

ChIP-chip: considerations for the design, analysis, and application of genome-wide chromatin immunoprecipitation experiments.

机译：ChIP芯片：设计，分析和应用全基因组染色质免疫沉淀实验的注意事项。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chromatin immunoprecipitation (ChIP) is a well-established procedure to investigate interactions between proteins and DNA. Coupled with whole-genome DNA microarrays, ChIPS allow one to determine the entire spectrum of in vivo DNA binding sites for any given protein. The design and analysis of ChIP-microarray (also called ChIP-chip) experiments differ significantly from the conventions used for locus ChIP approaches and ChIP-chip experiments, and these differences require new methods of analysis. In this light, we review the design of DNA microarrays, the selection of controls, the level of repetition required, and other critical parameters for success in the design and analysis of ChIP-chip experiments, especially those conducted in the context of mammalian or other relatively large genomes.
机译：染色质免疫沉淀（ChIP）是研究蛋白质与DNA之间相互作用的公认方法。结合全基因组DNA微阵列，ChIPS可以确定任何给定蛋白质的体内DNA结合位点的整个光谱。 ChIP微阵列（也称为ChIP芯片）实验的设计和分析与基因座ChIP方法和ChIP芯片实验所使用的惯例存在显着差异，这些差异需要新的分析方法。有鉴于此，我们回顾了DNA微阵列的设计，对照的选择，所需的重复水平以及成功设计和分析ChIP芯片实验的其他关键参数，尤其是在哺乳动物或其他背景下进行的实验相对较大的基因组。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号