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Identification of mirtrons in rice using MirtronPred: A tool for predicting plant mirtrons

机译：使用MirtronPred鉴定水稻中的mirtrons：一种预测植物mirtrons的工具

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Studies from flies and insects have reported the existence of a special class of miRNA, called mirtrons that are produced from spliced-out introns in a DROSHA-independent manner. The spliced-out lariat is debranched and refolded into a stem-loop structure resembling the pre-miRNA, which can then be processed by DICER into mature ~. 21 nt species. The mirtrons have not been reported from plants. In this study, we present MirtronPred, a web based server to predict mirtrons from intronic sequences. We have used the server to predict 70 mirtrons in rice introns that were put through a stringent selection filter to shortlist 16 best sequences. The prediction accuracy was subsequently validated by northern analysis and RT-PCR of a predicted Os-mirtron-109. The target sequences for this mirtron were also found in the rice degradome database. The possible role of the mirtron in rice regulon is discussed. The MirtronPred web server is available at http://bioinfo.icgeb.res.in/mirtronPred.
机译：来自苍蝇和昆虫的研究报告说，存在一类特殊的miRNA，称为mirtrons，它是由剪接的内含子以DROSHA独立的方式产生的。将剪接的套索解分支并重新折叠成类似于pre-miRNA的茎环结构，然后可以用DICER将其加工成成熟的〜。 21 nt种。尚未从植物中报道过mirtrons。在这项研究中，我们介绍了MirtronPred，这是一个基于Web的服务器，可以根据内含子序列预测mirtrons。我们已经使用该服务器预测了水稻内含子中的70个mirtron，它们通过严格的选择过滤器筛选出16个最佳序列。随后，通过预测的Os-mirtron-109的Northern分析和RT-PCR验证了预测的准确性。还可以在水稻降解组数据库中找到该mirtron的靶序列。讨论了mirtron在水稻调节子中的可能作用。可从http://bioinfo.icgeb.res.in/mirtronPred获得MirtronPred Web服务器。

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