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Transcriptome analysis of rosette and folding leaves in Chinese cabbage using high-throughput RNA sequencing

机译：高通量RNA测序技术对大白菜花环和折叠叶片的转录组分析

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摘要

In this study, we report the first use of RNA-sequencing to gain insight into the wide range of transcriptional events that are associated with leafy head development in Chinese cabbage. We generated 53.5. million sequence reads (90. bp in length) from the rosette and heading leaves. The sequence reads were aligned to the recently sequenced Chiifu genome and were analyzed to measure the gene expression levels, to detect alternative splicing events and novel transcripts, to determine the expression of single nucleotide polymorphisms, and to refine the annotated gene structures. The analysis of the global gene expression pattern suggests two important concepts, which govern leafy head formation. Firstly, some stimuli, such as carbohydrate levels, light intensity and endogenous hormones might play a critical role in regulating the leafy head formation. Secondly, the regulation of transcription factors, protein kinases and calcium may also be involved in this developmental process.
机译：在这项研究中，我们报告了首次使用RNA测序来深入了解与大白菜叶头发育相关的广泛转录事件。我们生成了53.5。玫瑰花序和抽穗叶中的数百万个序列读数（长度为90 bp）。将序列读数与最近测序的Chiifu基因组进行比对，并进行分析以测量基因表达水平，以检测替代的剪接事件和新的转录本，确定单核苷酸多态性的表达，并完善注释的基因结构。对全球基因表达模式的分析提出了两个重要的概念，它们控制着叶状头的形成。首先，一些刺激，例如碳水化合物水平，光照强度和内源激素可能在调节叶头的形成中起关键作用。其次，转录因子，蛋白激酶和钙的调节也可能参与该发育过程。

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