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Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation

机译：莱茵衣藻脂质积累过程中的转录组分析

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摘要

Algae are potential candidates for biodiesel production; thus, it is important to gain insight into the molecular mechanism of their lipid metabolism. Time-course transcriptome analyses were carried out during the lipid biosynthesis and accumulation processes of the model green alga Chlamydomonas reinhardtii using the Illumina RNA-seq platform. Transcriptome results indicated that over 2500 genes are upregulated or during lipid accumulation compared to log phase growth. As a proof of principle, two of the enzymes required for lipid metabolism that were significantly up-regulated during lipid accumulation, Lyso-Phosphatidic Acid Acyltransferase (LPAAT), diacylglycerol acyltransferase (DAGAT) were knocked down using artificial microRNAs. Neutral lipid production decreased in strains knocked down in expression of the lpaat and dagat genes. In addition, forty-one transcription factors were up- or down-regulated during the lipid accumulation process. This transcriptome data will be useful for engineering economic algae species aimed at biodiesel production.
机译：藻类是生物柴油生产的潜在候选人。因此，重要的是深入了解其脂质代谢的分子机制。使用Illumina RNA-seq平台在模型绿藻莱茵衣藻的脂质生物合成和积累过程中进行了时程转录组分析。转录组结果表明，与对数生长期相比，超过2500个基因上调或在脂质积累过程中。作为原理的证明，脂质代谢所需的两种在脂质积累过程中显着上调的酶，即溶血磷脂酸酰基转移酶（LPAAT），二酰基甘油酰基转移酶（DAGAT）使用人工microRNA敲低了。在lpaat和dagat基因表达被敲低的菌株中，中性脂质的产量下降。此外，在脂质蓄积过程中有41个转录因子上调或下调。该转录组数据将对工程化旨在生产生物柴油的经济藻类物种有用。

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