Refinement of the smallest commonly deleted segment of chromosome 20 in malignant myeloid diseases and development of a PAC-based physical and transcription map.

Wang PW; Eisenbart JD; Espinosa R; Davis EM; Larson RA; Le-Beau MM

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Refinement of the smallest commonly deleted segment of chromosome 20 in malignant myeloid diseases and development of a PAC-based physical and transcription map.

机译：在恶性骨髓疾病中细化20号染色体中通常最小的缺失片段，并开发基于PAC的物理和转录图。

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A deletion of the long arm of chromosome 20, del(20q), is a recurring abnormality in malignant myeloid diseases. In previous studies, we delineated a commonly deleted segment (CDS) of 5 Mb within band 20q12 flanked by D20S206 (proximal) and D20S481 (distal). We have generated a detailed physical map of P1 artificial chromosome (PAC) clones of this interval as well as a transcriptional map. The contig consists of 81 clones to which 152 markers (27 genes, 45 unique expressed sequence tags (ESTs) or UniGenes, 24 polymorphisms, and 56 sequence-tagged sites) have been mapped. Using PAC clones for fluorescence in situ hybridization analysis of myeloid leukemia cells with reciprocal translocations of 20q, or unbalanced rearrangements leading to loss of 20q, we have narrowed the CDS to an approximately 250-kb interval encompassing two overlapping PACs, P201E16 and P29M7 (between EST AA368224 and D20S481). This interval is gene-rich and contains 5 characterized genes, 4 UniGenes, and 9 single ESTs. The development of a transcriptional map and the identification of the smallest CDS will facilitate the molecular cloning of a myeloid leukemia suppressor gene on 20q.

机译：在恶性骨髓疾病中，第20号染色体长臂del（20q）的缺失是复发性异常。在以前的研究中，我们在20q12频段内划定了一个5 Mb的常见缺失片段（CDS），两侧是D20S206（近端）和D20S481（远端）。我们已经生成了该间隔的P1人工染色体（PAC）克隆的详细物理图以及转录图。重叠群由81个克隆组成，其中已绘制了152个标记（27个基因，45个独特的表达序列标签（EST）或UniGenes，24个多态性和56个序列标记的位点）。使用PAC克隆对髓系白血病细胞进行20q易位或不平衡重排导致20q丢失的荧光原位杂交分析，我们将CDS缩小到大约250kb的间隔，涵盖了两个重叠的PAC，P201E16和P29M7（之间EST AA368224和D20S481）。该间隔富含基因，包含5个特征基因，4个UniGene和9个单EST。转录图谱的发展和最小CDS的鉴定将有助于在20q上克隆髓样白血病抑制基因。

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《Genomics》 |2000年第1期|共12页
作者
Wang PW; Eisenbart JD; Espinosa R; Davis EM; Larson RA; Le-Beau MM;
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正文语种 eng
中图分类遗传学;
关键词
Chromosome Deletion; Chromosome Mapping; Chromosomes; Yeast Artificial; Leukemia; Myeloid; 染色体缺失; 染色体图; 染色体; 酵母人工; 白血病; 髓样;

机译：Chromosome Deletion;Chromosome Mapping;Chromosomes;Yeast Artificial;Leukemia;Myeloid;染色体缺失;染色体图;染色体;酵母人工;白血病;髓样;

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1. Refinement of the smallest commonly deleted segment of chromosome 20 in malignant myeloid diseases and development of a PAC-based physical and transcription map. [J] . Wang PW, Eisenbart JD, Espinosa R, Genomics . 2000,第1期

机译：在恶性骨髓疾病中细化20号染色体中通常最小的缺失片段，并开发基于PAC的物理和转录图。
2. Transcript map and comparative analysis of the 1.5-Mb commonly deleted segment of human 5q31 in malignant myeloid diseases with a del(5q). [J] . Lai F, Godley LA, Joslin J, Genomics . 2001,第2期

机译：带有del（5q）的恶性骨髓疾病中人类5q31 1.5-Mb常见缺失片段的转录图和比较分析。
3. Refinement within single yeast artificial chromosome clones of a minimal region commonly deleted on the short arm of chromosome 7 in Wilms tumours. [J] . Perotti D, Testi MA, Mondini P, Genes, Chromosomes and Cancer . 2001,第1期

机译：在单酵母人工染色体克隆中细化通常在Wilms肿瘤的7号染色体短臂上缺失的最小区域。
4. Physical and transcriptional characterization of a region on the short arm of chromosome 1 (1p21--p31): A novel locus implicated in hereditary malignant melanoma. [D] . Faruque, Mezbah Ul. 2003

机译：染色体1短臂（1p21--p31）上一个区域的物理和转录特征：与遗传性恶性黑色素瘤有关的新型基因座。
5. Molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases to 1–1.5 Mb and preparation of a PAC-based physical map [O] . Nanding Zhao, Archontoula Stoffel, Pauline W. Wang, 1997

机译：将恶性骨髓病中5号染色体上最小的通常缺失区域分子描绘为1–1.5 Mb并准备基于PAC的物理图谱
6. Molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases to 1-1.5 Mb and preparation of a PAC-based physical map [O] . N. Zhao, A. Stoffel, P. W. Wang, 1997

机译：恶性骨髓疾病中染色体5最小常见的染色体5的分子描绘至1-1.5 MB，并制备PAC的物理图

Refinement of the smallest commonly deleted segment of chromosome 20 in malignant myeloid diseases and development of a PAC-based physical and transcription map.

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