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首页> 外文期刊>Genes and Development: a Journal Devoted to the Molecular Analysis of Gene Expression in Eukaryotes, Prokaryotes, and Viruses >The piggyBac transposon-derived genes TPB1 and TPB6 mediate essential transposon-like excision during the developmental rearrangement of key genes in Tetrahymena thermophila
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The piggyBac transposon-derived genes TPB1 and TPB6 mediate essential transposon-like excision during the developmental rearrangement of key genes in Tetrahymena thermophila

机译：嗜热四膜膜球菌关键基因的发育重排过程中，piggyBac转座子衍生的基因TPB1和TPB6介导必需的转座子样切除。

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摘要

Ciliated protozoans perform extreme forms of programmed somatic DNA rearrangement during development. The model ciliate Tetrahymena thermophila removes 34% of its germline micronuclear genome from somatic macronuclei by excising thousands of internal eliminated sequences (IESs), a process that shares features with transposon excision. Indeed, piggyBac transposon-derived genes are necessary for genome-wide IES excision in both Tetrahymena (TPB2 [Tetrahymena piggyBac-like 2] and LIA5) and Paramecium tetraurelia (PiggyMac). T. thermophila has at least three other piggyBac-derived genes: TPB1, TPB6, and TPB7. Here, we show that TPB1 and TPB6 excise a small, distinct set of 12 unusual IESs that disrupt exons. TPB1-deficient cells complete mating, but their progeny exhibit slow growth, giant vacuoles, and osmotic shock sensitivity due to retention of an IES in the vacuolar gene DOP1 (Dopey domain-containing protein). Unlike most IESs, TPB1-dependent IESs have piggyBac-like terminal inverted motifs that are necessary for excision. Transposon-like excision mediated by TPB1 and TPB6 provides direct evidence for a transposon origin of not only IES excision machinery but also IESs themselves. Our study highlights a division of labor among ciliate piggyBac-derived genes, which carry out mutually exclusive categories of excision events mediated by either transposon-like features or RNA-directed heterochromatin.
机译：有纤毛的原生动物在发育过程中会执行编程形式的体细胞DNA重排的极端形式。该模型纤毛虫四膜膜虫通过切除数千个内部消除序列（IESs），从体细胞大核中去除了其种系微核基因组的34％，这一过程与转座子切除具有共同的特征。的确，在四膜虫（TPB2 [四膜虫piggyBac样2]和LIA5）和四草履虫（PiggyMac）中，piggyBac转座子衍生的基因对于全基因组IES切除是必需的。嗜热链球菌还有至少三个其他的piggyBac衍生基因：TPB1，TPB6和TPB7。在这里，我们显示TPB1和TPB6切除了12个不寻常的小IES，它们破坏了外显子。缺乏TPB1的细胞完全交配，但由于IES保留在液泡基因DOP1（含Dopey域的蛋白质）中，它们的后代表现出缓慢的生长，巨大的液泡和渗透压休克敏感性。与大多数IES不同，与TPB1相关的IES具有切除所需的类似piggyBac的末端倒置基序。由TPB1和TPB6介导的类似转座子的切除不仅为IES切除机制而且为IES本身提供了转座子起源的直接证据。我们的研究突出了纤毛piggyBac衍生基因之间的分工，这些基因执行互斥类的由转座子样特征或RNA定向异染色质介导的切除事件。

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