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首页> 外文期刊>Genes & Genetic Systems >Characterization of a fission yeast P-5-type ATPase homologue that is essential for Ca2+/Mn2+ homeostasis in the absence of P-2-type ATPases
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Characterization of a fission yeast P-5-type ATPase homologue that is essential for Ca2+/Mn2+ homeostasis in the absence of P-2-type ATPases

机译：裂变酵母P-5型ATPase同源物的表征，在没有P-2-型ATPase的情况下对于Ca2 + / Mn2 +稳态至关重要

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摘要

In the fission yeast Schizosaccharomyces pombe, three P-type ATPases, namely Cta4p, Pmr1p, and Pmc1p, have been shown to be essential for Ca2+ homeostasis and are required for specific cellular functions as well. Here, we show that the simultaneous deletion of pmc1(+) and SPAC29A4.19c, which encodes a putative P-5-type ATPase, causes a hypersensitive growth to either high concentrations of Ca2+ in a medium, or the antiarrhythmic drug amiodarone, which has been known to cause a disruption of Ca2+ homeostasis. On the other hand, simultaneous deletion of pmr1(+) and SPAC29A4.19c causes a hypersensitive growth to Mn2+ depletion in a medium. The green fluorescent protein-tagged SPAC29A4.19c protein reveals a typical localization pattern of the Golgi proteins, but the SPAC29A4.19c protein is not exchangeable in function with Pmr1p, which is required for Ca2+/Mn2+ homeostasis in secretory pathways. These results suggest that the putative P5-type ATPase encoded by SPAC29A4.19c is essential for Ca2+ and Mn2+ homeostasis in the absence of P-2-type ATPases, Pmc1p or Pmr1p, respectively. According to the precedent nomenclature of calcium/cation transporting ATPase in fission yeast, SPAC29A4.19 was named cta5(+) in this study.
机译：在裂殖酵母粟酒裂殖酵母中，三种P型ATP酶Cta4p，Pmr1p和Pmc1p已被证明对Ca2 +稳态至关重要，并且对于特定的细胞功能也是必需的。在这里，我们显示同时缺失pmc1（+）和SPAC29A4.19c（编码一种假定的P-5型ATPase）会导致对培养基中高浓度Ca2 +或抗心律不齐药物胺碘酮的过敏反应。已知会破坏Ca2 +稳态。另一方面，同时删除pmr1（+）和SPAC29A4.19c会导致培养基中Mn2 +耗竭过度敏感。带有绿色荧光蛋白标签的SPAC29A4.19c蛋白显示出高尔基蛋白的典型定位模式，但SPAC29A4.19c蛋白不能与Pmr1p交换功能，而Pmr1p是分泌途径中Ca2 + / Mn2 +稳态所需的。这些结果表明，在没有P-2-型ATPase，Pmc1p或Pmr1p的情况下，由SPAC29A4.19c编码的假定的P5-型ATPase对于Ca2 +和Mn2 +稳态是必不可少的。根据裂变酵母中钙/阳离子转运ATPase的先例命名法，在本研究中将SPAC29A4.19命名为cta5（+）。

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