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首页> 外文期刊>Genes & Genetic Systems >Microsatellite markers derived from Quercus mongolica var. crispula (Fagaceae) inner bark expressed sequence tags.
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Microsatellite markers derived from Quercus mongolica var. crispula (Fagaceae) inner bark expressed sequence tags.

机译：来自蒙古栎的微卫星标记。薄脆菌（Fagaceae）内树皮表达的序列标签。

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摘要

In reforestation programs the genetic composition and diversity of populations that could be used as sources of planting material needs to be carefully considered to maximize the chances of successful establishment. For such purposes genetic analyses that include the identification of functional genes are required. In this study, we constructed a cDNA library from inner bark of Quercus mongolica (which is widely distributed in Japan) and collected 3385 ESTs. After constructing 2140 unigenes, 274 microsatellites were found within them. The most frequent microsatellite had AG motif (48%) and the next most common was AAG motif (12%). There were no CG repeats in the unigenes. In total, 20 EST-SSR markers were developed, polymorphisms of which were described by using eight individuals from eight populations over the species' distributional range. The number of alleles per locus (Na) and observed heterozygosity (H(o)) ranged from 2 to 12, and from 0.25 to 1.00, respectively. Cross-species amplification was successful for 19 loci in eight individuals of Q. serrata and for 20 loci in eight individuals of Q. dentata, with values of Na and H(o) comparable to those of Q. mongolica. The EST-SSR markers characterized in this study should facilitate the analysis of genetic diversity in future studies.
机译：在重新造林计划中，需要仔细考虑可以用作种植材料来源的种群的遗传组成和多样性，以最大程度地提高成功定植的机会。为此，需要包括功能基因鉴定在内的遗传分析。在这项研究中，我们从蒙古栎（在日本广泛分布）的内部树皮构建了一个cDNA文库，并收集了3385个EST。构建2140个单基因后，在其中发现274个微卫星。最常见的微卫星具有AG主题（48％），其次最常见的是AAG主题（12％）。单基因中没有CG重复。总共开发了20种EST-SSR标记，通过使用来自该物种分布范围的8个种群的8个个体来描述其多态性。每个基因座的等位基因数（Na）和观察到的杂合度（H（o））分别为2至12和0.25至1.00。跨物种扩增成功完成了锯齿拟南芥的八个个体中的19个位点和齿状拟南芥的八个个体中的20个基因座的Na和H（o）值可与蒙古Q.相媲美。这项研究中表征的EST-SSR标记应有助于将来研究中遗传多样性的分析。

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